Medical Basic Experimental Teaching Center, China Medical University, Shenyang 110122, China.
Clinical Laboratory, PLA North Military Command Region General Hospital, Shenyang 110003, China.
Biomed Res Int. 2022 May 14;2022:7798654. doi: 10.1155/2022/7798654. eCollection 2022.
Accumulating evidence demonstrated that FOXD1 dysregulation was correlated with a broad spectrum of malignancies. However, litter is known about the role of FOXD1 in the progression of lung squamous cell carcinoma (LUSC). We conducted the comprehensive bioinformatics analysis to investigate FOXD1 expression in LUSC from TCGA and GEO datasets, and validated the FOXD1 expression pattern in clinical samples using immunohistochemistry method. ESTIMATE and CIBERSORT algorithms were performed to assess the relationship of FOXD1 and tumor microenvironment and immune cell infiltration. Our study showed that FOXD1 expression was significantly upregulated in LUSC tissues in TCGA dataset, validated by GEO datasets and clinical samples. In TCGA dataset, Kaplan-Meier curves showed that high FOXD1 expression was significantly correlated with favorable prognosis in LUSC patients. Moreover, FOXD1 expression has an impact on immune score and the proportions of immune cell infiltration subgroups. Finally, we predicted FOXD1 may be involved in many immune-related biological functions and cancer-related signaling pathways. Taken together, FOXD1 was upregulated in LUSC tissues, and FOXD1 expression could be a potential prognostic marker. FOXD1 might be associated with tumor microenvironment and perhaps a potential target in the tumor immunotherapy.
越来越多的证据表明，FOXD1 的失调与广泛的恶性肿瘤有关。然而，关于 FOXD1 在肺鳞状细胞癌（LUSC）进展中的作用知之甚少。我们对 TCGA 和 GEO 数据集的 LUSC 中的 FOXD1 表达进行了全面的生物信息学分析，并通过免疫组织化学方法在临床样本中验证了 FOXD1 的表达模式。ESTIMATE 和 CIBERSORT 算法用于评估 FOXD1 与肿瘤微环境和免疫细胞浸润的关系。我们的研究表明，FOXD1 在 TCGA 数据集中的 LUSC 组织中表达显著上调，这一结果在 GEO 数据集和临床样本中得到了验证。在 TCGA 数据集中，Kaplan-Meier 曲线表明，高 FOXD1 表达与 LUSC 患者的良好预后显著相关。此外，FOXD1 表达对免疫评分和免疫细胞浸润亚群的比例有影响。最后，我们预测 FOXD1 可能参与许多免疫相关的生物学功能和癌症相关的信号通路。综上所述，FOXD1 在 LUSC 组织中上调，FOXD1 表达可能是一个潜在的预后标志物。FOXD1 可能与肿瘤微环境有关，也许是肿瘤免疫治疗的一个潜在靶点。
