Guangdong Province Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
Food Funct. 2022 Jun 6;13(11):6282-6292. doi: 10.1039/d2fo01056a.
The aims of this study were to evaluate whether a diet supplemented with glyceryl butyrate could attenuate the immune-inflammatory response in piglets challenged with enterotoxigenic (ETEC), and to explore the mechanisms of its regulation. Eighteen weaning piglets were assigned to three diets: basal diet (CON), antibiotics diet (ATB), and 0.5% glyceryl butyrate diet (GB group). Significantly lower concentrations of IL-1β, IL-6 and TNF-α in the jejunum and IL-6 in the ileum were observed in the GB group than that in the CON group ( < 0.05). Moreover, a decreasing trend of IL-1β ( = 0.075) and TNF-α ( = 0.070) was observed in the ileum in the GB group. Correspondingly, the GB group had significantly increased mRNA expression of porcine beta defensins (pBDs) in the jejunum (pBD1, pBD2, pBD114 and pBD129) and ileum (pBD2, pBD3, pBD114 and pBD129) ( < 0.05), and protein abundance of Claudin 1, Occludin, and ZO-1 in the jejunum and ileum ( < 0.05). Further research results showed that the improvement of beta defensins and tight junctions in the GB group was related to the decreased phosphorylation of the NFκB/MAPK pathway. In addition, the results of 16S rDNA sequencing showed that glycerol butyrate supplementation altered the ileal microbiota composition of piglets, increasing the relative abundance of , , and . In summary, glyceryl butyrate attenuated the immune-inflammatory response in piglets challenged with ETEC by inhibiting the NF-κB/MAPK pathways and modulating the gut microbiota, and thus improved piglet intestinal health.
本研究旨在评估甘油丁酸酯日粮是否可以减轻肠产毒性大肠杆菌（ETEC）攻毒仔猪的免疫炎症反应，并探讨其调控机制。将 18 头断奶仔猪分为 3 组：基础日粮组（CON）、抗生素日粮组（ATB）和 0.5%甘油丁酸酯日粮组（GB 组）。与 CON 组相比，GB 组仔猪空肠和回肠中 IL-1β、IL-6 和 TNF-α的浓度显著降低（<0.05），且 GB 组回肠中 IL-1β（=0.075）和 TNF-α（=0.070）呈下降趋势。GB 组仔猪空肠和回肠中猪β防御素（pBD）的 mRNA 表达显著增加（pBD1、pBD2、pBD114 和 pBD129）（<0.05），空肠和回肠中 Claudin1、Occludin 和 ZO-1 的蛋白丰度也显著增加（<0.05）。进一步的研究结果表明，GB 组 pBD 和紧密连接的改善与 NF-κB/MAPK 通路磷酸化的降低有关。此外，16S rDNA 测序结果显示，甘油丁酸酯的添加改变了仔猪回肠微生物群的组成，增加了 、 、和 的相对丰度。综上所述，甘油丁酸酯通过抑制 NF-κB/MAPK 通路和调节肠道微生物群来减轻 ETEC 攻毒仔猪的免疫炎症反应，从而改善仔猪肠道健康。
