Itoh K, Lee W K, Kawamura H, Mitsuoka T, Magaribuchi T
Lab Anim. 1987 Jan;21(1):20-5. doi: 10.1258/002367787780740662.
Overgrowth by Clostridium difficile has been reported in conventional mice injected intraperitoneally with ampicillin. In this study, we aimed to determine which types of indigenous intestinal bacteria were eliminated by ampicillin to allow overgrowth by C. difficile. C. difficile overgrowth was associated with a decrease in the numbers of lactobacilli, an increase in bacteroidaceae and a slight decrease in the frequency of isolation of fusiform-shaped bacteria (clostridia). C. difficile cytotoxin was detected in caeca from mice in which the numbers of C. difficile were greater than 10(5) per gram of faeces. Gnotobiotic mice were inoculated with various groups of intestinal anaerobes to determine which members of the indigenous flora would antagonize C. difficile. Gnotobiotic mice inoculated with three strains of lactobacilli, 37 strains of bacteroides or 46 strains of clostridia isolated from limited-flora mice were unable to eliminate C. difficile. C. difficile was eliminated, however, from the gastrointestinal tracts of gnotobiotic mice inoculated with whole faeces or chloroform-treated faeces from conventional mice or whole faeces from limited-flora mice containing only clostridia.
据报道，腹腔注射氨苄青霉素的传统小鼠中艰难梭菌过度生长。在本研究中，我们旨在确定氨苄青霉素消除了哪些类型的肠道固有细菌，从而使得艰难梭菌得以过度生长。艰难梭菌过度生长与乳酸杆菌数量减少、拟杆菌科数量增加以及梭形细菌（梭菌）分离频率略有降低有关。在每克粪便中艰难梭菌数量大于10⁵的小鼠盲肠中检测到艰难梭菌细胞毒素。给无菌小鼠接种不同组别的肠道厌氧菌，以确定固有菌群中的哪些成员会拮抗艰难梭菌。接种从有限菌群小鼠分离出的三株乳酸杆菌、37株拟杆菌或46株梭菌的无菌小鼠无法清除艰难梭菌。然而，接种来自传统小鼠的全粪便或经氯仿处理的粪便或仅含梭菌的有限菌群小鼠的全粪便后，艰难梭菌从无菌小鼠的胃肠道中被清除。
