柿叶黄酮磷脂复合物对动脉粥样硬化的作用及可能机制。
Efficacy of phospholipid complex of flavonoids from persimmon leaves on atherosclerosis, and possible mechanism.
机构信息
State Key Laboratory of Drug Delivery and Pharmacokinetics, Tianjin 300301, China.
2 Tianjin Key Laboratory of TCM quality markers, Tianjin 300301, China.
出版信息
J Tradit Chin Med. 2022 Jun;42(3):417-425. doi: 10.19852/j.cnki.jtcm.2022.03.008.
OBJECTIVE
To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves (PLF-PC) on atherosclerosis, and to study its mechanism behind the action.
METHODS
To clarify the constituents of the flavonoids from persimmon leaves (PLF), an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method was established. To enhance the anti-atherosclerotic effect of PLF, a newly emerging approach based on the combination of phospholipid complexation technique was employed. PLF-PC was prepared by the solvent-evaporation method then characterized using Fourier transform infrared spectroscopy, Powder X-Ray Diffractometry and Scanning electron microscopy. A model of oxidized low-density lipoprotein-induced injury on human umbilical vein endothelial cells was established to investigate the anti-atherosclerotic effect of PLF-PC versus PLF. The levels of nitric oxide, endothelial nitric oxide synthase, intracellular adhesion molecule-1, reactive oxygen species, superoxide dismutase, tumor necrosis factor-αand nuclear factor-κB were observed assay kits.
RESULTS
A total of 31 compounds were identified in PLF. PLF-PC showed better anti-atherosclerotic power compared with PLF, moreover, enzyme linked immune-osorbent assay analysis showed that the PLF-PC may effect on endothelial dysfunction and atherosclerosis antioxidant-related mechanisms.
CONCLUSIONS
Our findings elucidated that PLF-PC significantly enhanced the PLF's efficacy on atherosclerosis.
目的
研究柿叶类黄酮磷脂复合物（PLF-PC）对动脉粥样硬化的疗效，并研究其作用机制。
方法
为了阐明柿叶中类黄酮（PLF）的成分，建立了超高效液相色谱/四极杆飞行时间质谱法。为了增强 PLF 的抗动脉粥样硬化作用，采用了一种新兴的基于磷脂复合物化技术结合的方法。采用溶剂蒸发法制备 PLF-PC，并用傅里叶变换红外光谱、粉末 X 射线衍射和扫描电子显微镜对其进行表征。建立了氧化型低密度脂蛋白诱导的人脐静脉内皮细胞损伤模型，以研究 PLF-PC 与 PLF 对动脉粥样硬化的抗动脉粥样硬化作用。通过试剂盒观察一氧化氮、内皮型一氧化氮合酶、细胞间黏附分子-1、活性氧、超氧化物歧化酶、肿瘤坏死因子-α和核因子-κB 的水平。
结果
在 PLF 中鉴定出 31 种化合物。PLF-PC 显示出比 PLF 更强的抗动脉粥样硬化作用，此外，酶联免疫吸附分析表明，PLF-PC 可能作用于内皮功能障碍和动脉粥样硬化抗氧化相关机制。
结论
我们的研究结果表明，PLF-PC 显著增强了 PLF 对动脉粥样硬化的疗效。
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