Department of Reproduction, Poznan University of Medical Sciences, 61-701, Poznan, Poland.
Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 61-701, Poznan, Poland.
Sci Rep. 2022 May 24;12(1):8696. doi: 10.1038/s41598-022-12221-5.
Despite improvement in the care of diabetes over the years, pregnancy complicated by type 1 diabetes (T1DM) is still associated with adverse maternal and neonatal outcomes. To date, proteomics studies have been conducted to identify T1DM biomarkers in non-pregnant women, however, no studies included T1DM pregnant women. In this study serum proteomic profiling was conducted in pregnant women with T1DM in the late third trimester. Serum samples were collected from 40 women with T1DM and 38 healthy controls within 3 days before delivery at term pregnancy. Significant differences between serum proteomic patterns were revealed, showing discriminative peaks for complement C3 and C4-A, kininogen-1, and fibrinogen alpha chain. Quantification of selected discriminative proteins by ELISA kits was also performed. The serum concentration of kininogen-1 was significantly lower in women with T1DM than in controls. There were no significant differences in serum concentrations of complement C3 and complement C4-A between study groups. These data indicate that pregnant women with T1DM have a distinct proteomic profile involving proteins in the coagulation and inflammatory pathways. However, their utility as biomarkers of pregnancy complications in women with T1DM warrants further investigation.
尽管多年来糖尿病的治疗得到了改善，但妊娠合并 1 型糖尿病（T1DM）仍然与母婴不良结局相关。迄今为止，已经进行了蛋白质组学研究来鉴定非妊娠女性的 T1DM 生物标志物，但没有研究包括 T1DM 孕妇。在这项研究中，对妊娠晚期的 T1DM 孕妇进行了血清蛋白质组学分析。在足月妊娠前 3 天内，从 40 名 T1DM 孕妇和 38 名健康对照者中采集了血清样本。揭示了血清蛋白质图谱之间存在显著差异，补体 C3 和 C4-A、激肽原-1 和纤维蛋白原α链具有有区别的峰。还通过 ELISA 试剂盒对选定的有区别蛋白进行了定量。T1DM 孕妇的血清激肽原-1浓度明显低于对照组。两组间补体 C3 和补体 C4-A 的血清浓度无显著差异。这些数据表明，T1DM 孕妇具有涉及凝血和炎症途径的蛋白质的独特蛋白质组学特征。然而，它们作为 T1DM 孕妇妊娠并发症的生物标志物的效用还需要进一步研究。
