一种基于功能化氧化石墨烯、木质素、丝素蛋白和 ZnO 纳米粒子的新型生物活性抗菌支架。

A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles.

机构信息

Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran.

Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

出版信息

Sci Rep. 2022 May 24;12(1):8770. doi: 10.1038/s41598-022-12283-5.

In this study, a novel nanobiocomposite was synthesized using graphene oxide, lignin, silk fibroin and ZnO and used in biological fields. To synthesize this structure, after preparing graphene oxide by the Hummer method, lignin, silk fibroin, and ZnO nanoparticles (NPs) were added to it, respectively. Also, ZnO NPs with a particle size of about 18 nm to 33 nm was synthesized via Camellia sinensis extract by green methodology. The synthesized structure was examined as anti-biofilm agent and it was observed that the Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite has a significant ability to prevent the formation of P. aeruginosa biofilm. In addition, due to the importance of the possibility of using this structure in biological environments, its toxicity and blood compatibility were also evaluated. According to the obtained results from MTT assay, the viability percentages of Hu02 cells treated with Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite after 24, 48, and 72 h of incubation were 89.96%, 89.32%, and 91.28%. On the other hand, the hemolysis percentage of the synthesized structure after 24 h and 72 h of extraction was 9.5% and 11.76% respectively. As a result, the synthesized structure has a hemolysis percentage below 12% and its toxicity effect on Hu02 cells is below 9%.

在这项研究中，使用氧化石墨烯、木质素、丝素蛋白和 ZnO 合成了一种新型的纳米生物复合材料，并将其应用于生物领域。为了合成这种结构，首先通过 Hummer 法制备氧化石墨烯，然后分别向其中加入木质素、丝素蛋白和 ZnO 纳米粒子（NPs）。此外，还通过绿茶提取物采用绿色方法合成了粒径约为 18nm 至 33nm 的 ZnO NPs。研究人员将合成的结构作为抗生物膜剂进行了检测，结果表明氧化石墨烯-木质素/丝素蛋白/ZnO 纳米生物复合材料具有显著的抑制铜绿假单胞菌生物膜形成的能力。此外，由于该结构在生物环境中具有应用的可能性，还对其毒性和血液相容性进行了评估。根据 MTT 测定的结果，在孵育 24、48 和 72 小时后，用氧化石墨烯-木质素/丝素蛋白/ZnO 纳米生物复合材料处理的 Hu02 细胞的存活率分别为 89.96%、89.32%和 91.28%。另一方面，合成结构在提取 24 小时和 72 小时后的溶血率分别为 9.5%和 11.76%。因此，合成结构的溶血率低于 12%，对 Hu02 细胞的毒性作用低于 9%。