脑脊液去甲变肾上腺素的液相色谱测定法
Liquid chromatographic assay for cerebrospinal fluid normetanephrine.
作者信息
Marshall T H, Jacobson K A, Kirk K L, Linnoila M
出版信息
Life Sci. 1987 Apr 13;40(15):1513-21. doi: 10.1016/0024-3205(87)90384-5.
A method for quantitation of normetanephrine in human cerebrospinal fluid is described. An amine-specific reagent, sulfosuccinimidyl propionate, is used to obtain the lipid soluble N-propionyl derivative of normetanephrine, which can be separated and quantitated in presence of other biogenic amines by liquid chromatography with electrochemical detection. The method is reproducible, linear, and precise at the relatively low concentrations of unconjugated normetanephrine occurring in human cerebrospinal fluid. Hospitalized, drug-free, alcoholic patients were found to have cerebrospinal fluid unconjugated normetanephrine concentrations in the 0.5-1.5 nanomolar range. The practical limit of sensitivity for the method is about 0.025 pmole per ml of CSF.
本文描述了一种定量测定人脑脊液中去甲变肾上腺素的方法。使用一种胺特异性试剂——磺基琥珀酰亚胺丙酸酯，来获得去甲变肾上腺素的脂溶性N-丙酰基衍生物，该衍生物可在其他生物胺存在的情况下，通过液相色谱-电化学检测进行分离和定量。该方法在人脑脊液中未结合的去甲变肾上腺素相对较低浓度下具有可重复性、线性和精确性。研究发现，住院的、未服用药物的酒精性患者脑脊液中未结合的去甲变肾上腺素浓度在0.5至1.5纳摩尔范围内。该方法的实际灵敏度极限约为每毫升脑脊液0.025皮摩尔。
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