N-羟基琥珀酰亚胺酯酰化后生物胺的电化学检测。
Electrochemical detection of biogenic amines following acylation by N-hydroxysuccinimide esters.
作者信息
Jacobson K A, Marshall T, Mine K, Kirk K L, Linnoila M
出版信息
FEBS Lett. 1985 Sep 2;188(2):307-11. doi: 10.1016/0014-5793(85)80392-6.
Abstract
A general methodology for the selective derivatization of amines, to enable quantitation by high pressure liquid chromatography with electrochemical detection, is presented. N-Hydroxysuccinimide active esters present in large excess are suitably mild acylating agents to derivatize selectively trace quantities of amines for electrochemical detection. The 2 separate problems of extraction yield and detectability can be solved by this derivatization method. Due to its lipophilicity the resulting N-acylated amine, as demonstrated with serotonin, is extracted efficiently into organic solvents during sample preparation for chromatography. Moreover, the acyl group introduced can be designed to be electroactive, thus extending the procedure to amines not readily oxidized, e.g., histamine and phenylethylamine.
摘要
本文介绍了一种用于胺类选择性衍生化的通用方法，该方法能够通过带有电化学检测的高压液相色谱进行定量分析。大量过量存在的N-羟基琥珀酰亚胺活性酯是合适的温和酰化剂，可用于选择性衍生痕量胺类以进行电化学检测。这种衍生化方法可以解决萃取产率和可检测性这两个独立的问题。如血清素所示，由于其亲脂性，所得的N-酰化胺在色谱样品制备过程中能有效地萃取到有机溶剂中。此外，引入的酰基可设计为具有电活性，从而将该方法扩展到不易氧化的胺类，如组胺和苯乙胺。
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