Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Department of Orthopedics Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Bioengineered. 2022 May;13(5):13082-13088. doi: 10.1080/21655979.2022.2078025.
To explore the molecular mechanism of cartilage calcification induced by osteoarthritis (OA) based on distal-less homeobox gene 5 - alkaline phosphatase - integrin-binding sialoprotein - ecto-nucleotide pyrophosphatase 1 (DLX5-ALPL-IBSP-ENPP1) signal axis. Twenty-four rabbits were selected to build models of cartilage calcification induced by OA and randomly divided into 3 groups. The first group was the normal group whose rabbits were injected into 0.9% saline (0.3 mL), and the second group was model group. The third group was model group whose rabbits were injected into DLX5 antibody by caudal vein. Alizarin red calcium staining was used to analyze calcium deposition of cartilage matrix. Immunohistochemical staining was used to analyze the relative expression levels of proteins DLX5 and ENPP1, and western blot was used to analyze the DLX5, ALPL, IBSP, and ENPP1 expression. Calcium salt precipitation was the most serious, and the calcification area increased in the model group. Although calcified nodules appeared in the anti-DLX5 group, they were relatively few. Immunohistochemical staining analysis showed that the protein DLX5 located in the nucleus and the protein ENPP1 located in the extracellular matrix. Western blot analysis showed that the expressions of proteins DLX5, ALPL, IBSP, and ENPP1 were the highest in OA Model group than that of NC group, followed by anti-DLX5 group. The proteins DLX5, ALPL, IBSP, and ENPP1 can promote cartilage calcification induced by OA based on DLX5-ALPL-IBSP-ENPP1 signal axis.
探讨基于远侧板状同源框基因 5-碱性磷酸酶-整合素结合唾液蛋白-外核苷酸焦磷酸酶 1（DLX5-ALPL-IBSP-ENPP1）信号轴的骨关节炎（OA）诱导软骨钙化的分子机制。选择 24 只兔子建立 OA 诱导的软骨钙化模型，并随机分为 3 组。第 1 组为正常组，兔子注射 0.9%生理盐水（0.3 mL）；第 2 组为模型组；第 3 组为模型组，尾静脉注射 DLX5 抗体。茜素红钙染色分析软骨基质钙沉积，免疫组化染色分析蛋白 DLX5 和 ENPP1 的相对表达水平，Western blot 分析 DLX5、ALPL、IBSP 和 ENPP1 的表达。模型组钙盐沉淀最严重，钙化面积增加。虽然抗-DLX5 组出现钙化结节，但相对较少。免疫组化染色分析显示，蛋白 DLX5 位于细胞核内，蛋白 ENPP1 位于细胞外基质中。Western blot 分析显示，OA 模型组中蛋白 DLX5、ALPL、IBSP 和 ENPP1 的表达均高于 NC 组，其次是抗-DLX5 组。蛋白 DLX5、ALPL、IBSP 和 ENPP1 可通过 DLX5-ALPL-IBSP-ENPP1 信号轴促进 OA 诱导的软骨钙化。
