College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, China.
Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou, China.
Anal Methods. 2022 Jun 9;14(22):2188-2194. doi: 10.1039/d2ay00384h.
is one of the major foodborne pathogens, which may cause serious food safety problems and illnesses in humans and animals. Consequently, sensitive, fast and reliable detection methods, as well as effective sample preparation methods are in great demand. In this study, a magnetic separation method based on a aptamer functionalized positively-charged magnetic beads (FeO@aptamer) was established and a fluorescent test strip sensor was constructed for the rapid, sensitive and specific detection of . Benefiting from the dual recognition and signal amplification process of FeO@aptamer enrichment and the polymerase chain reaction of the gene, the fluorescent strip sensor for the detection of was determined to be reliable and sensitive, with a linear curve obtained in the range of 1.0 × 10 to 1.9 × 10 CFU mL, and a detection limit of 1.0 × 10 CFU mL. The detection was achieved in 3 h without culture enrichment. Furthermore, the developed method was successfully applied for the detection of in pork tenderloin, with the recoveries ranging from 91.1% to 97.1%, and a coefficient of variation of less than 23.4%, revealing the feasible and reliable application of this method in practical samples. The proposed fluorescent strip sensor is rapid, sensitive and specific, giving it great application prospects for use in the field of pathogenic bacterium detection.
是一种主要的食源性致病菌，可能会导致人类和动物的严重食品安全问题和疾病。因此，人们迫切需要灵敏、快速和可靠的检测方法以及有效的样品制备方法。在本研究中，建立了一种基于适配体功能化正电荷磁性珠（FeO@aptamer）的磁分离方法，并构建了一种荧光检测试纸传感器，用于快速、灵敏和特异性检测。得益于 FeO@aptamer 富集的双重识别和信号放大过程以及基因的聚合酶链式反应，用于检测的荧光试纸传感器被证明是可靠和灵敏的，在 1.0×10 到 1.9×10 CFU mL 的范围内获得了线性曲线，检测限为 1.0×10 CFU mL。无需培养富集即可在 3 小时内完成检测。此外，该方法成功应用于猪肉里脊中 的检测，回收率在 91.1%至 97.1%之间，变异系数小于 23.4%，表明该方法在实际样品中具有可行且可靠的应用。所提出的荧光检测试纸传感器快速、灵敏且具有特异性，在病原菌检测领域具有广阔的应用前景。
