Surekha Challa, Srikanth Racha, Thupurani Murali Krishna, Neelapu Nageswara Rao Reddy, Peddireddy Vidyullatha
Department of Biochemistry and Bioinformatics, Institute of Science, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh, 530045, India.
Department of Biotechnology, Chaitanya (Deemed to be University), Kishanpura, Hanamkonda, Warangal, Telangana, India.
Curr Microbiol. 2022 May 25;79(7):204. doi: 10.1007/s00284-022-02888-4.
Antibiotic resistance and the hazardous nature of synthetic drugs is threatening issue in the health sector. The alternative for this problem is to focus on plants that attribute to various compounds that exhibit therapeutic properties. Therefore, the study aims to evaluate the antimicrobial efficacy of Salacia oblonga leaf and root extracts against tested human pathogens. The S. oblonga extracts showed a significant zone of inhibition against bacteria and fungi. The leaf and root extracts of S. oblonga are prepared using low polar to high polar solvents in the Soxhlet apparatus and tested on the selected bacterial and fungal strains. Agar well diffusion and broth dilution methods evaluate antibacterial activity, antifungal activity, and Minimum Inhibitory Concentration (MIC) of extracts. Among the extracts tested, the ethyl acetate extract of root showed more antimicrobial activity against the tested bacterial and fungal strains. The most susceptible bacterial and fungal species against ethyl acetate extract are Micrococcus luteus, Mycobacterium tuberculosis, Microsporum canis, Trichophyton interdigitale, and Microsporum gypseum. The MIC for bacteria ranged from 13.0 to > 200 µg/ml, whereas for fungi, the MIC ranged from 25.9 to > 200 µg/ml. Ethyl acetate extract of root with 100 µg/ml concentration showed 29.1 mm and 28.7 mm zone of inhibition against bacterial strains M. luteus and M. tuberculosis, respectively. The ethyl acetate extract of root with a 100 µg/ml concentration showed 15.8, 15.2, and 15.6 mm zone of inhibition against fungal isolates M. canis, T. interdigitale, and M. gypseum, respectively. The activity of root and leaf extracts increased in a concentration-dependent manner, and further, the compounds isolated from the crude extracts of leaf and root showed antimicrobial activity. Structural elucidation of isolated compounds Lambertic acid and Ferruginol was done using NMR spectroscopy. Reports indicate that Lambertic acid was isolated previously, but the isolation of hydroxy Ferruginol from S. oblonga leaf extract was reported unprecedented.
抗生素耐药性以及合成药物的有害性质是卫生部门面临的一个严峻问题。解决这个问题的替代方法是关注那些含有具有治疗特性的各种化合物的植物。因此，本研究旨在评估椭圆叶刺蒴麻叶和根提取物对受试人类病原体的抗菌效果。椭圆叶刺蒴麻提取物对细菌和真菌显示出显著的抑菌圈。椭圆叶刺蒴麻的叶和根提取物是在索氏提取器中使用从低极性到高极性的溶剂制备的，并在选定的细菌和真菌菌株上进行测试。采用琼脂孔扩散法和肉汤稀释法评估提取物的抗菌活性、抗真菌活性和最低抑菌浓度（MIC）。在所测试的提取物中，根的乙酸乙酯提取物对受试细菌和真菌菌株表现出更强的抗菌活性。对乙酸乙酯提取物最敏感的细菌和真菌物种是藤黄微球菌、结核分枝杆菌、犬小孢子菌、指间毛癣菌和石膏样小孢子菌。细菌的MIC范围为13.0至>200μg/ml，而真菌的MIC范围为25.9至>200μg/ml。浓度为100μg/ml的根乙酸乙酯提取物对藤黄微球菌和结核分枝杆菌菌株的抑菌圈分别为29.1mm和28.7mm。浓度为100μg/ml的根乙酸乙酯提取物对犬小孢子菌、指间毛癣菌和石膏样小孢子菌真菌分离株的抑菌圈分别为15.8mm、15.2mm和15.6mm。根和叶提取物的活性呈浓度依赖性增加，此外，从叶和根的粗提物中分离出的化合物也显示出抗菌活性。使用核磁共振光谱对分离出的化合物兰伯酸和铁杉醇进行了结构解析。报告表明，兰伯酸以前已被分离出来，但从椭圆叶刺蒴麻叶提取物中分离出羟基铁杉醇的情况此前未见报道。
