Sun Yuwei, Zhang Shiyao, Li Hong, Zhu Jiang, Liu Zhijia, Hu Xiaosong, Yi Junjie
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Front Microbiol. 2022 May 9;13:895132. doi: 10.3389/fmicb.2022.895132. eCollection 2022.
The lack of rapid and effective approaches to determine the health benefits of strains is one of the main challenges affecting the selection of probiotics from large numbers of candidates. In this study, the probiotic potential of 44 strains isolated from different Chinese traditional fermented foods was evaluated, including acid and bile salt resistance, adhesion ability, survival in simulated human gastrointestinal transit, antioxidant activity, bile salt hydrolase (BSH) activity, and antibacterial activity. All tested strains showed high antioxidant capacity, BSH activity, and antibacterial activity. Among the strains, B652, C232, D444, and E932 were identified as the best comprehensive performed strains, which were selected for whole-genome sequencing, in order to provide clear information and identify key genes responsible for functional characteristics . It demonstrated that the antioxidant activity, adhesion activity, and ability to survive in the simulated gastric environment were found to be closely correlated with antioxidant enzyme encoding genes, cell-surface protein-encoding genes, and stress response genes, respectively. The numbers of functional genes present in strains might decide their performance in probiotic profile evaluation. The outcome of the study could support the development of a novel approach for the screening and identification of probiotics.
缺乏快速有效的方法来确定菌株的健康益处是影响从大量候选菌株中选择益生菌的主要挑战之一。在本研究中，评估了从不同中国传统发酵食品中分离出的44株菌株的益生菌潜力，包括耐酸和耐胆盐能力、黏附能力、在模拟人体胃肠道转运中的存活率、抗氧化活性、胆盐水解酶（BSH）活性和抗菌活性。所有测试菌株均表现出高抗氧化能力、BSH活性和抗菌活性。在这些菌株中，B652、C232、D444和E932被鉴定为综合表现最佳的菌株，对其进行全基因组测序，以便提供清晰信息并鉴定负责功能特性的关键基因。结果表明，抗氧化活性、黏附活性和在模拟胃环境中的存活能力分别与抗氧化酶编码基因、细胞表面蛋白编码基因和应激反应基因密切相关。菌株中存在的功能基因数量可能决定其在益生菌特性评估中的表现。该研究结果可为开发一种新型益生菌筛选和鉴定方法提供支持。
