Zhang Lihong, Ma Hongcai, Kulyar Muhammad Fakhar-E-Alam, Pan Huachun, Li Kewei, Li Aoyun, Mo Quan, Wang Yaping, Dong Hailong, Bao Yuhua, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Tibet Livestock Research Institute, Tibet Academy of Agriculture And Animal Science, Lhasa 850009, Tibet, People's Republic of China.
Microb Pathog. 2022 Jan;162:105212. doi: 10.1016/j.micpath.2021.105212. Epub 2021 Sep 28.
Lactobacillus fermentum (L. fermentum) YLF016 is a well-characterized probiotic with several favorable characteristics. This study aimed to analyze the probiotic characteristics of L. fermentum and uncover the genes implicated in its potential probiotic ability on the base of its genomics features. The complete genome of L. fermentum YLF016 was found to have a circular chromosome of 2,094,354 bp, and 51.46% G + C content without any plasmid. Its chromosome contained 2,130 predicted protein-encoding genes, 58 tRNA, and 15 rRNA-encoding genes. Also, it was found to have many other probiotic properties, such as a high survival rate in the gastrointestinal tract with strong adherence to intestinal cells, antibacterial activity against pathogens, and antioxidant activity. Moreover, the genome sequence analysis demonstrated specific genes coding for carbon metabolism pathway, genetic adaption, stress resistance, and adhesive ability. Further analysis revealed its non-hemolytic activity and its non-functional ability of virulence factors. In conclusion, L. fermentum YLF016 possesses many valuable probiotic properties that refer to its potential probiotic ability.
发酵乳杆菌（L. fermentum）YLF016是一种具有多种优良特性的、特征明确的益生菌。本研究旨在分析发酵乳杆菌的益生菌特性，并基于其基因组特征揭示与其潜在益生菌能力相关的基因。研究发现，发酵乳杆菌YLF016的完整基因组具有一条2,094,354 bp的环状染色体，G + C含量为51.46%，且不含任何质粒。其染色体包含2,130个预测的蛋白质编码基因、58个tRNA和15个rRNA编码基因。此外，还发现它具有许多其他益生菌特性，如在胃肠道中的高存活率、对肠道细胞的强粘附性、对病原体的抗菌活性和抗氧化活性。此外，基因组序列分析显示了编码碳代谢途径、遗传适应、抗逆性和粘附能力的特定基因。进一步分析揭示了其非溶血活性和毒力因子的无功能能力。总之，发酵乳杆菌YLF016具有许多有价值的益生菌特性，这表明了其潜在的益生菌能力。
