Zhejiang Pharmaceutical College, Ningbo 315100, Zhejiang Province, PR China.
Ningbo University School of Medicine, Ningbo 315211, China.
Food Funct. 2022 Jun 6;13(11):6419-6432. doi: 10.1039/d2fo00952h.
Osteoporosis (OP) is characterized by the flaccidity of bones or bone bi-disease caused by kidney deficiency. has been used to strengthen kidney function in China for thousands of years. It has been approved by Chinese Pharmacopoeia that the root of (RLA) can replenish and tonify the kidney, which is thought to be an effective way to alleviate OP. In this study, a network pharmacology approach was applied to explore the active components and potential mechanisms of RLA in osteoporosis treatment. Then, the ethanolic extract of the root of (EERL) was prepared and these predicted results were validated by prednisone-induced zebrafish embryos model. Moreover, the candidate compounds were identified by UPLC-ESI-MS/MS. The anti-OP results showed that EERL could significantly reverse the bone loss of zebrafish induced by prednisone. The mRNA expressions results showed that EERL decreased osteoclast bone resorption by regulating the RANK/RANKL/OPG system. Also, it increased bone formation by regulating the gene expressions of , , , , , and . Our results demonstrated the reliability of the network pharmacology method, and also revealed the anti-OP effect and potential mechanism of RLA.
骨质疏松症（OP）的特征是骨骼松弛或由肾虚引起的骨骼双疾病。在中国，已经使用 数千年来增强肾功能。中国药典已经批准， 的根（RLA）可以补肾填精，这被认为是缓解 OP 的有效方法。在这项研究中，应用网络药理学方法探讨 RLA 在骨质疏松症治疗中的活性成分和潜在机制。然后，制备了 的根的乙醇提取物（EERL），并通过泼尼松诱导的斑马鱼胚胎模型验证了这些预测结果。此外，通过 UPLC-ESI-MS/MS 鉴定候选化合物。抗 OP 结果表明，EERL 可显著逆转泼尼松诱导的斑马鱼骨丢失。mRNA 表达结果表明，EERL 通过调节 RANK/RANKL/OPG 系统减少破骨细胞的骨质吸收，同时通过调节 、 、 、 、 和 的基因表达增加骨形成。我们的结果证明了网络药理学方法的可靠性，同时揭示了 RLA 的抗 OP 作用和潜在机制。
