Instituto Politécnico Nacional, CIIDIR Unidad Sinaloa, Guasave, Sinaloa, Mexico.
Plant Foods Hum Nutr. 2022 Jun;77(2):265-270. doi: 10.1007/s11130-022-00977-0. Epub 2022 May 26.
α-tocopherol is found in high concentrations in avocado fruit mesocarp, however, its accumulation and genetic control during maturation and ripening has not been elucidated. Based in the relevance of VTE1 and VTE5 genes in tocopherol biosynthesis and aiming to determine the association between tocopherol accumulation and expression of tocopherol biosynthetic genes, gene expression of VTE1 and VTE5 were evaluated through the time during three developmental stages: before harvest at 100, 160 and 220 days after flowering (DAF) and after harvest (220 DAF + 5) in two contrasting avocado genotypes (San Miguel and AVO40). San Miguel reached the highest levels at 220 DAF, whereas AVO40 increased α-tocopherol only after ripening (220 DAF + 5). A genome-wide search for VTE1 and VTE5 allowed to identify one and three genes, respectively. Both genotypes showed contrasting patterns of gene expression. Interestingly, AVO40 showed a highly positive correlation between α-tocopherol levels and gene expression of VTE1 and all VTE5 variants. On the other hand, San Miguel showed only a positive correlation between α-tocopherol level and VTE1gene expression.
α-生育酚在鳄梨果实中果皮中含量很高，但在成熟和软化过程中的积累和遗传控制尚未阐明。基于 VTE1 和 VTE5 基因在生育酚生物合成中的重要性，以及旨在确定生育酚积累与生育酚生物合成基因表达之间的关联，本研究通过三个发育阶段（100、160 和 220 天授粉后）和收获后（220 DAF+5）评估了 VTE1 和 VTE5 的基因表达，在两个不同的鳄梨基因型（San Miguel 和 AVO40）中进行。San Miguel 在 220 DAF 时达到最高水平，而 AVO40 仅在成熟后（220 DAF+5）才增加 α-生育酚。对 VTE1 和 VTE5 的全基因组搜索分别鉴定到一个和三个基因。两个基因型表现出不同的基因表达模式。有趣的是，AVO40 中 α-生育酚水平与 VTE1 基因和所有 VTE5 变体的基因表达呈高度正相关。另一方面，San Miguel 中仅观察到 α-生育酚水平与 VTE1 基因表达之间存在正相关。
