环丙氨嗪通过减少成年雌性卵巢中的生殖细胞数量来影响其繁殖。
Cyromazine Effects the Reproduction of by Decreasing the Number of Germ Cells in the Female Adult Ovary.
作者信息
Khalid Muhammad Zaryab, Sun Zhipeng, Chen Yaoyao, Zhang Jing, Zhong Guohua
机构信息
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
出版信息
Insects. 2022 Apr 27;13(5):414. doi: 10.3390/insects13050414.
In the present study, we observed a 58% decrease in the fecundity of , after treatment with the cyromazine. To further elucidate the effects of cyromazine on reproduction, we counted the number of both germline stem cells (GSCs) and cystoblasts (CBs) in the ovary of a 3-day-old adult female. The results showed a significant decrease in the number of GSCs and CBs as compared to the control group. The mode of action of cyromazine is believed to be through the ecdysone signaling pathway. To further support this postulate, we observed the expression of key genes involved in the ecdysone signaling pathway and also determined the ecdysone titer from cyromazine-treated ovaries. Results indicated a significant decrease in the expression of ecdysone signaling-related genes as compared to the control group. Furthermore, the titer of the ecdysone hormone was also markedly reduced (90%) in cyromazine-treated adult ovaries, suggesting that ecdysone signaling was directly related to the decrease in the number of GSCs and CBs. However, further studies are required to understand the mechanism by which cyromazine affects the GSCs and CBs in female adult ovaries.
在本研究中，我们观察到用环丙氨嗪处理后，其繁殖力下降了58%。为了进一步阐明环丙氨嗪对生殖的影响，我们对3日龄成年雌性卵巢中的生殖系干细胞（GSCs）和成囊母细胞（CBs）的数量进行了计数。结果显示，与对照组相比，GSCs和CBs的数量显著减少。环丙氨嗪的作用方式被认为是通过蜕皮激素信号通路。为了进一步支持这一假设，我们观察了蜕皮激素信号通路中关键基因的表达，并测定了经环丙氨嗪处理的卵巢中的蜕皮激素滴度。结果表明，与对照组相比，蜕皮激素信号相关基因的表达显著降低。此外，在经环丙氨嗪处理的成年卵巢中，蜕皮激素的滴度也显著降低（90%），这表明蜕皮激素信号与GSCs和CBs数量的减少直接相关。然而，需要进一步的研究来了解环丙氨嗪影响成年雌性卵巢中GSCs和CBs的机制。
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