UCSF, Department of Anatomy, 513 Parnassus Ave, San Francisco, CA, 94143, USA.
UCSF, Department of OB-GYN/RS, 513 Parnassus Ave, San Francisco, CA, 94143, USA.
Nat Commun. 2020 Nov 6;11(1):5628. doi: 10.1038/s41467-020-19361-0.
The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.
果蝇卵巢是研究生殖细胞和体组织生物学的广泛应用模型。在这里，我们使用单细胞 RNA 测序（scRNA-seq）构建了成年果蝇卵巢的综合细胞图谱，其中包含了卵巢中每种主要细胞类型的转录谱，包括生殖干细胞及其生态位细胞、滤泡干细胞，以及以前未被描述的 escort 细胞亚群。此外，我们还鉴定了具有特定表达模式的 Gal4 系，并对 escort 细胞和滤泡细胞的亚群进行了谱系追踪。我们发现，在饥饿或遗传操作（包括 escargot 的敲低，或 mTor 或 Toll 信号的过度激活）的情况下，一个独特的 escort 细胞亚群能够转化为滤泡干细胞。
