原发性卵巢癌肿瘤组织及其转移灶中 microRNA 基因甲基化水平的协同性。
Synergy between the Levels of Methylation of microRNA Gene Sets in Primary Tumors and Metastases of Ovarian Cancer Patients.
机构信息
Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2022 May;173(1):87-91. doi: 10.1007/s10517-022-05499-y. Epub 2022 May 27.
We studied the correlations between the levels of methylation of a group of 21 microRNA genes in 99 primary tumors and 29 macroscopic peritoneal metastases of ovarian cancer. Analysis of the level of methylation by quantitative methylation-specific PCR showed that co-methylation was detected for 13 pairs of microRNA genes in primary tumors and for 22 pairs in metastases. Pairs of microRNA genes that have shown significant co-methylation can be involved in common processes and pathways of gene regulation and interaction and can have common target genes. The results are highly significant and pairs of microRNA genes can be proposed as new potential markers for the diagnosis and prognosis of ovarian cancer metastasis.
我们研究了 99 个原发性卵巢癌肿瘤和 29 个宏观腹膜转移灶中一组 21 个 microRNA 基因的甲基化水平之间的相关性。通过定量甲基化特异性 PCR 分析甲基化水平的结果显示，在原发性肿瘤中检测到 13 对 microRNA 基因的共甲基化，在转移灶中检测到 22 对。表现出显著共甲基化的 microRNA 基因对可能参与共同的基因调控和相互作用过程，并可能具有共同的靶基因。这些结果具有高度显著性，microRNA 基因对可以作为卵巢癌转移诊断和预后的新的潜在标志物。
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