AIRR 社区 AIRR-Seq 实验规划和执行指南
AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.
机构信息
Center for Regenerative Therapies Dresden, Faculty of Medicine, TU Dresden, Dresden, Germany.
Takara Bio USA, San Jose, CA, USA.
出版信息
Methods Mol Biol. 2022;2453:261-278. doi: 10.1007/978-1-0716-2115-8_15.
The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.
高通量测序技术的发展，为深入分析免疫系统提供了一个新的前沿领域，即适应性免疫受体库（IG 和 TR 重排的 AIRR-seq）。在过去的十年中，出现了大量的协议、实验方法和计算工具。在本章中，我们讨论了成功进行 AIRR-seq 实验的主要注意事项，以及控制和评估实验结果的基本策略。AIRR 社区的成员在这一版中撰写了几章，涵盖了成功进行、分析和共享 AIRR-seq 项目的逐步说明。
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