University Children's Hospital and the Children's Research Center, University of Zurich, Zurich, Switzerland.
CRTD Center for Regenerative Therapies Dresden, Faculty of Medicine, Technische Universität Dresden, Dresden, Germany.
Elife. 2021 May 26;10:e66274. doi: 10.7554/eLife.66274.
Use of adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread, providing new insights into the immune system with potential broad clinical and diagnostic applications. However, like many high-throughput technologies, it comes with several problems, and the AIRR Community was established to understand and help solve them. We, the AIRR Community's Biological Resources Working Group, have surveyed scientists about the need for standards and controls in generating and annotating AIRR-seq data. Here, we review the current status of AIRR-seq, provide the results of our survey, and based on them, offer recommendations for developing AIRR-seq standards and controls, including future work.
使用适应性免疫受体库测序（AIRR-seq）已经变得非常普遍，为免疫系统提供了新的见解，并具有广泛的临床和诊断应用潜力。然而，与许多高通量技术一样，它也存在一些问题，因此建立了 AIRR 社区来理解和帮助解决这些问题。我们，AIRR 社区的生物资源工作组，已经调查了科学家们在生成和注释 AIRR-seq 数据方面对标准和对照的需求。在这里，我们回顾了 AIRR-seq 的现状，提供了我们的调查结果，并在此基础上，为制定 AIRR-seq 标准和对照提出了建议，包括未来的工作。
