Ramos-Zayas Yareellys, Cantú-Reyes Saúl A, Tristán-Casas Iris I, Kawas Jorge R
Universidad Autónoma de Nuevo León, Posgrado Conjunto Agronomía-Veterinaria, Avenida Francisco Villa S/N, Colonia Ex-Hacienda El Canadá, Escobedo 66050, Nuevo León, Mexico.
MNA de México, Avenida Acapulco 770, Colonia La Fe, San Nicolás de los Garza 66477, Nuevo León, Mexico.
Vet Sci. 2022 May 11;9(5):230. doi: 10.3390/vetsci9050230.
Most in vivo studies related to ruminal development in calves use invasive techniques involving rumen-fistulated or euthanized animals. In consideration of animal welfare, we developed an oral endoscopic biopsy procedure to allow the obtaining of rumen epithelial samples, thus serving as an alternative for measuring the height and width of rumen papillae in calves in a safe, quick, and efficient manner that allows the slaughtering of calves to be avoided. This procedure was tested on 12 Brangus crossbred calves randomly distributed in two groups, with one fed a meal starter and the other an extruded starter feed. Calves underwent a 12-h fasting period, were restrained in a squeeze chute, administered a dose of atropine, and sedated with xylazine before the oral endoscopic biopsy procedure. A 120 cm long Olympus oral flexible video endoscope and forceps were used to collect cranial-dorsal sac rumen epithelial tissue samples of approximately 0.5 mm. Endoscopy was successful in all 12 calves and the collected tissue samples were placed in formalin (10%) for further processing for obtaining rumen papillae measurements. Consumption of the extruded starter feed resulted in the increased ( = 0.035) width of rumen papillae. The oral endoscopic biopsy procedure implemented in this study was demonstrated to be successful and is thus an alternative technique for studying rumen epithelial development and morphometric alterations in calf rumen tissue.
大多数与犊牛瘤胃发育相关的体内研究都采用了侵入性技术，涉及瘤胃造瘘或安乐死的动物。考虑到动物福利，我们开发了一种口腔内镜活检程序，以便获取瘤胃上皮样本，从而以安全、快速且高效的方式替代测量犊牛瘤胃乳头的高度和宽度，避免对犊牛进行屠宰。该程序在12头随机分为两组的勃兰格斯杂交犊牛上进行了测试，一组饲喂粉状开食料，另一组饲喂挤压型开食料。在进行口腔内镜活检程序之前，犊牛禁食12小时，被限制在挤压式畜栏中，注射一剂阿托品，并使用赛拉嗪进行镇静。使用一根120厘米长的奥林巴斯口腔柔性视频内窥镜和镊子采集约0.5毫米的瘤胃背囊头侧上皮组织样本。12头犊牛的内镜检查均成功，采集的组织样本置于10%的福尔马林中，以便进一步处理以测量瘤胃乳头。食用挤压型开食料导致瘤胃乳头宽度增加（ = 0.035）。本研究中实施的口腔内镜活检程序被证明是成功的，因此是研究犊牛瘤胃组织中瘤胃上皮发育和形态计量学改变的一种替代技术。
