Hayat Khizar, Raza Ali, Anas Aitzaz, Qureshi Anas Sarwar, Rehan Sarmad, Rabbani Ameer Hamza, Rehman Hafiz Faseeh Ur, Qamar Abdul Ghaffar, Rehman Tayyab, Deeba Farah, Salman Amber
Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong 999077, China.
Department of Anatomy, University of Agriculture, Faisalabad 38000, Pakistan.
Vet Sci. 2022 May 23;9(5):248. doi: 10.3390/vetsci9050248.
The present study was designed to elucidate a relationship between lymphoid organs and reproductive activity in male Japanese quails () bred in a temperate region of Pakistan (30.3753° N, 69.3451° E) in response to photoperiodic changes. The research focused primarily on the relative morphological changes in primary (thymus and bursa of Fabricius) and secondary (spleen) lymphoid organs with respect to seasonal variations in the histomorphometry of testicular tissue. For this purpose, a comparable number of clinically healthy Japanese quails were exsanguinated during active (April-May), regressive (September-October) and inactive (January-February) reproductive phases. Following an extensive gross measurement of lymphoid and reproductive organs, a histomorphometric analysis was performed on sampled tissues by employing ImageJ software. Blood was collected for hormonal and leukocytic analysis. One-way ANOVA was used for statistical comparison. Testes had the highest parenchymal development in the active phase (80.66 ± 21.22 µm) and the lowest in the inactive phase (27.80 ± 7.22 µm). Conversely, a percentage change was evident in the sizes of primary (bursa: 61.5%, thymus: 46.9%) and secondary (spleen: 23.9%) lymphoid organs during inactive and active reproductive phases. This study demonstrated that a physiological trade-off is imperative between immune and reproductive systems for optimum survivability and reproductive performance.
本研究旨在阐明在巴基斯坦温带地区（北纬30.3753°，东经69.3451°）饲养的雄性日本鹌鹑中，淋巴器官与生殖活动之间的关系，以应对光周期变化。该研究主要关注初级（胸腺和法氏囊）和次级（脾脏）淋巴器官相对于睾丸组织组织形态计量学季节性变化的相对形态变化。为此，在活跃（4月至5月）、退化（9月至10月）和非活跃（1月至2月）生殖阶段，对数量相当的临床健康日本鹌鹑进行放血。在对淋巴和生殖器官进行广泛的大体测量之后，通过使用ImageJ软件对采样组织进行组织形态计量学分析。采集血液进行激素和白细胞分析。使用单因素方差分析进行统计比较。睾丸实质在活跃期发育最高（80.66±21.22µm），在非活跃期最低（27.80±7.22µm）。相反，在非活跃和活跃生殖阶段，初级（法氏囊：61.5%，胸腺：46.9%）和次级（脾脏：23.9%）淋巴器官的大小有明显的百分比变化。这项研究表明，为了实现最佳的生存能力和生殖性能，免疫和生殖系统之间必须进行生理权衡。
