Akarsu Gokhan Dogukan, Akarsu Rukiye Hobek
Laboratory for Advanced Genomics, Division of Molecular Medicine, Institute of Ruder Boskovic, Zagreb, Croatia.
Pharmacy Services, Vocational School of Health Services, Yozgat Bozok University, Yozgat, Turkiye.
Vet Med (Praha). 2024 Aug 16;69(8):297-306. doi: 10.17221/21/2024-VETMED. eCollection 2024 Aug.
This study was conducted to evaluate the wound-healing activities of a L. plant extract and cannabidiol on incision wounds. An incision was created and sutured in rats under anaesthesia. Routine wound care procedures were applied for 10 days, followed by histological wound examinations. The cellular bioactivities of the hemp extract and CBD were assessed for MCP-1, EGF, BFGF, IL-8, and COL-1 using ELISA on the rat skin wound healing activity. A one-way ANOVA was used for the data analysis. The EGF values in the plasma were similar in the povidone-iodine, hemp seed oil, and hemp essential oil groups ( > 0.05). However, the EGF levels were lower in the CBD group compared to the other groups ( < 0.001, < 0.005). The MCP-1 values in the hemp seed oil, hemp essential oil, and CBD were similar ( > 0.05), whereas povidone iodine exhibited lower MCP-1 levels compared to the other groups ( 0.001, < 0.005). It was determined that the plasma BFGF, IL-8, and COL 1 values of the groups were similar ( > 0.05). To our knowledge, this study is the first to evaluate the effects of CBD, seed oil, and hemp leaf extract on incision wound healing. It demonstrates that hemp extract holds greater potential benefits for wound healing compared to CBD.
本研究旨在评估一种L.植物提取物和大麻二酚对切口伤口的愈合活性。在麻醉状态下对大鼠进行切口并缝合。进行10天的常规伤口护理程序，随后进行伤口组织学检查。使用酶联免疫吸附测定法（ELISA）评估大麻提取物和大麻二酚对大鼠皮肤伤口愈合活性中MCP-1、表皮生长因子（EGF）、碱性成纤维细胞生长因子（BFGF）、白细胞介素-8（IL-8）和I型胶原蛋白（COL-1）的细胞生物活性。采用单因素方差分析进行数据分析。聚维酮碘、大麻籽油和大麻精油组血浆中的EGF值相似（>0.05）。然而，与其他组相比，大麻二酚组的EGF水平较低（<0.001，<0.005）。大麻籽油、大麻精油和大麻二酚中的MCP-1值相似（>0.05），而聚维酮碘的MCP-1水平低于其他组（<0.001，<0.005）。结果确定各组的血浆BFGF、IL-8和COL 1值相似（>0.05）。据我们所知，本研究是首次评估大麻二酚、种子油和大麻叶提取物对切口伤口愈合的影响。结果表明，与大麻二酚相比，大麻提取物在伤口愈合方面具有更大的潜在益处。
