纳米臭氧化油处理的 B-16 黑色素瘤和 OV-90 卵巢细胞通过 ROS 产生诱导的电离辐射诱导的 DNA 损伤。

Ionizing radiation induced DNA damage via ROS production in nano ozonized oil treated B-16 melanoma and OV-90 ovarian cells.

机构信息

Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Incivez, Zonguldak, 67100, Turkey.

Department of Immunology, Zonguldak Bülent Ecevit University, School of Medicine, Zonguldak, 67100, Turkey.

出版信息

Biochem Biophys Res Commun. 2022 Jul 30;615:143-149. doi: 10.1016/j.bbrc.2022.05.030. Epub 2022 May 13.

DOI:10.1016/j.bbrc.2022.05.030

PMID:35623299

Abstract

In this study, we aimed to investigate ozonized oil nanoemulsions (OZNEs) as a radiosensitizer within B-16 melanoma and OV-90 ovarian cells under X-ray irradiation in vitro. Radiation sensitivity of OZNE treated B-16 melanoma cells and OV-90 ovarian cells were evaluated by performing cell cycle analysis, Reactive Oxygen Species (ROS) and ɣ-H2AX assays by flow cytometry. OZNEs induced G0-1 phase arrest of B-16 melanoma cells for all radiation doses and G2/M arrest for 8 Gy and 15 Gy doses. OZNE treated B-16 melanoma and OV-90 ovarian cells induced DNA damage via the increase in ROS production, as well as significant increase in the expression of ɣ-H2AX under even low doses of radiation (2 Gy). Thus, OZNEs are suggested to help to optimize cancer RT as a radiosensitizer and further studies will significantly outperform recent advances in this field.

摘要

在这项研究中，我们旨在研究臭氧油纳米乳液（OZNE）作为 X 射线照射下体外 B-16 黑色素瘤和 OV-90 卵巢细胞的放射增敏剂。通过细胞周期分析、流式细胞术检测活性氧（ROS）和γ-H2AX 分析来评估 OZNE 处理的 B-16 黑色素瘤细胞和 OV-90 卵巢细胞的辐射敏感性。OZNE 诱导所有辐射剂量的 B-16 黑色素瘤细胞 G0-1 期阻滞，8Gy 和 15Gy 剂量诱导 G2/M 期阻滞。OZNE 处理的 B-16 黑色素瘤和 OV-90 卵巢细胞通过增加 ROS 产生诱导 DNA 损伤，并且即使在低剂量辐射（2Gy）下，γ-H2AX 的表达也显著增加。因此，OZNE 被认为有助于优化癌症 RT 作为放射增敏剂，进一步的研究将显著超越该领域的最新进展。