Kianmehr Pantea, Azarbayjani Mohammad Ali, Peeri Maghsoud, Farzanegi Parvin
-Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
-Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
Biochem Biophys Rep. 2020 Feb 26;22:100735. doi: 10.1016/j.bbrep.2020.100735. eCollection 2020 Jul.
Octopamine (OCT) have an adverse effect on heart function. One of the positive effects of exercise training is improving cardiac function and cardiomyocytes signaling, which along with herbal supplements can have better effects on the heart tissue. Therefore, the aim of this study was to evaluate the effects of exercise training and OCT on changes of and expression in heart tissue of rat treated with deep frying oil (DFO). In this study, 45 male wistar rats were divided into 5 groups (n = 9 in each): I) control (Co), II) DFO, III) DFO + exercise, IV) DFO + OCT, and V) DFO + OCT + exercise. The quantification of apoptotic effects of DFO in heart tissue was assessed by TUNEL assay. Masson's trichrome stain applied to study cardiomyocytic fibers. Moreover, and genes and proteins expression in all groups were investigated using quantitative real-time PCR and immunohistochemical method. A significant increase in apoptotic cells was observed in the DFO-treated group (p < 0.05). In Masson's Trichrome stain study, more cardiomyocytic fibers were observed and some lymphocytic cells were present in some fibers. Also, the expression of and was significantly increase in DFO + exercise group, DFO + OCT group, and DFO + OCT + exercise group compare to DFO group (p < 0.05). Based on these findings, exercise and octopamine can be considered as factors affecting the expression of genes and as well as drug poisoning.
章鱼胺（OCT）对心脏功能有不良影响。运动训练的积极作用之一是改善心脏功能和心肌细胞信号传导，这与草药补充剂一起对心脏组织可能有更好的效果。因此，本研究的目的是评估运动训练和OCT对用煎炸油（DFO）处理的大鼠心脏组织中[未提及的两个基因或蛋白名称]表达变化的影响。在本研究中，45只雄性Wistar大鼠被分为5组（每组n = 9）：I）对照组（Co），II）DFO组，III）DFO + 运动组，IV）DFO + OCT组，V）DFO + OCT + 运动组。通过TUNEL法评估DFO对心脏组织的凋亡作用定量。应用Masson三色染色法研究心肌纤维。此外，使用定量实时PCR和免疫组化方法研究所有组中[未提及的两个基因或蛋白名称]的基因和蛋白表达。在DFO处理组中观察到凋亡细胞显著增加（p < 0.05）。在Masson三色染色研究中，观察到更多的心肌纤维，并且在一些纤维中存在一些淋巴细胞。此外，与DFO组相比，DFO + 运动组、DFO + OCT组和DFO + OCT + 运动组中[未提及的两个基因或蛋白名称]的表达显著增加（p < 0.05）。基于这些发现，运动和章鱼胺可被视为影响[未提及的两个基因或蛋白名称]基因表达以及药物中毒的因素。
