Shao Lingmei, Xu Tong, Wang Xiaobin, Zhang Runlong, Wang Xiuyun, Ren Ziming, Zhang Jiaping, Xia Yiping, Li Danqing
Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Antioxidants (Basel). 2022 May 16;11(5):977. doi: 10.3390/antiox11050977.
Cold acclimation (CA) is a strategy which plants have evolved to increase freezing tolerance. Global climate change could obstruct CA and raise the probability of winter injury, especially for evergreens. Hence, understanding the regulatory mechanism of CA is crucial to improve freezing tolerance in evergreen plants. A comparative study on a pair of closely related evergreen and deciduous iris species in response to cold through CA was conducive to uncovering and complementing the knowledge of CA. We investigated morphological, physiological and biochemical changes, as well as the expression of associated genes in the functional leaves of both iris species from natural CA to deacclimation. Briefly, fast and strong CA in the evergreen iris might cause early expressions of , , , etc., which leads to strong enzyme activity of starch degradation, abscisic acid biosynthesis and reactive oxygen species scavenging. Additionally, genes belonging to the antioxidant system were mainly induced during deacclimation. These results suggest that interspecies differences in the leaf freezing tolerance of irises are associated with the rate and degree of CA, which activates multiple signaling networks with complex interactions and induces the transcription of cold-responsive genes. Moreover, the ICE-CBF-COR signaling cascade may integrate and initiate diverse cold-responsive pathways during CA of the evergreen iris. The findings of this study provide valuable insight to further research on CA mechanisms and implicate genes which could support breeding strategies in herbaceous perennials under climate changes.
低温驯化（CA）是植物进化出的一种提高抗冻性的策略。全球气候变化可能会阻碍低温驯化并增加冬季伤害的可能性，尤其是对于常绿植物而言。因此，了解低温驯化的调控机制对于提高常绿植物的抗冻性至关重要。对一对亲缘关系密切的常绿和落叶鸢尾品种响应低温驯化的比较研究，有助于揭示和补充关于低温驯化的知识。我们研究了两种鸢尾品种从自然低温驯化到去驯化过程中，功能叶的形态、生理和生化变化以及相关基因的表达。简而言之，常绿鸢尾中快速且强烈的低温驯化可能会导致[此处原文缺失相关基因名称]等基因的早期表达，这会导致淀粉降解、脱落酸生物合成和活性氧清除的酶活性增强。此外，属于抗氧化系统的基因主要在去驯化过程中被诱导。这些结果表明，鸢尾叶片抗冻性的种间差异与低温驯化的速率和程度有关，低温驯化激活了具有复杂相互作用的多个信号网络，并诱导了冷响应基因的转录。此外，ICE-CBF-COR信号级联可能在常绿鸢尾的低温驯化过程中整合并启动多种冷响应途径。本研究结果为进一步研究低温驯化机制提供了有价值的见解，并暗示了一些基因，这些基因可为气候变化下多年生草本植物的育种策略提供支持。
