Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany.
Methods Mol Biol. 2020;2156:1-7. doi: 10.1007/978-1-0716-0660-5_1.
This introductory chapter provides a brief overview of plant freezing tolerance, cold acclimation, including subzero acclimation, and the subsequent deacclimation when plants return to warm conditions favoring growth and development. We describe the basic concepts and approaches that are currently followed to investigate these phenomena. We highlight the multidisciplinary nature of these investigations and the necessity to use methodologies from different branches of science, such as ecology, genetics, physiology, cell biology, biochemistry, and biophysics to gain a complete understanding of the complex adaptive mechanisms ultimately underlying plant winter survival.
本章简要概述了植物的抗冻性、低温驯化（包括亚零驯化），以及当植物返回有利于生长和发育的温暖条件时的随后脱驯化。我们描述了目前用于研究这些现象的基本概念和方法。我们强调了这些研究的多学科性质，以及必须使用来自生态学、遗传学、生理学、细胞生物学、生物化学和生物物理学等不同科学分支的方法学，以全面了解最终支持植物冬季生存的复杂适应机制。
