Lin Meng-Jin, Lin Ping, Wen Kuo-Ching, Chiang Hsiu-Mei, Lu Mei-Chun
Miaoli District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Miaoli 363-201, Taiwan.
Department of Cosmeceutics, China Medical University, Taichung 406-040, Taiwan.
Antioxidants (Basel). 2022 May 17;11(5):981. doi: 10.3390/antiox11050981.
Antioxidant and anti-inflammatory activities of Makino extract (FAE) on Hs68 fibroblasts and BALB/c nude-mouse models are evaluated in this study. FAE was found to be non-toxic and showed high levels of DPPH, HO, and hydroxyl radical scavenging abilities; a ferrous chelating capacity; as well as ferric-reducing antioxidant capability. The antioxidant activity of FAE was strongly associated with polyphenolic content (flavonoids at 10.3 mg QE g and total phenol at 107.6 mg GAE g). The anti-inflammatory activity of FAE and the underlying molecular mechanisms were also investigated. The a* value of the mouse dorsal skin after treatment with FAE at 1.5 mg/mL in addition to chronic UVB exposure was found to decrease by 19.2% during a ten-week period. The anti-inflammatory effect of FAE was evidenced by the decreased accumulation of inflammatory cells and skin thickness. Expression levels of UVB-induced inflammatory proteins, including ROS, NF-κB, iNOS, COX-2, and IL-6, were significantly reduced upon FAE treatment in vitro and in vivo. Collectively, our results suggest that the inhibition of ROS and UVB-induced activation of the NF-κB downstream signaling pathway by FAE, indicating considerable potential as a versatile adjuvant against free radical damage in pharmaceutical applications.
本研究评估了牧野提取物（FAE）对Hs68成纤维细胞和BALB/c裸鼠模型的抗氧化和抗炎活性。发现FAE无毒，并表现出高水平的DPPH、HO和羟自由基清除能力；亚铁螯合能力；以及铁还原抗氧化能力。FAE的抗氧化活性与多酚含量（黄酮类化合物为10.3 mg QE/g，总酚为107.6 mg GAE/g）密切相关。还研究了FAE的抗炎活性及其潜在的分子机制。在慢性紫外线B照射的同时，用1.5 mg/mL的FAE处理小鼠背部皮肤，发现其a*值在十周内下降了19.2%。炎症细胞积累和皮肤厚度的减少证明了FAE的抗炎作用。在体外和体内，FAE处理后，紫外线B诱导的炎症蛋白（包括ROS、NF-κB、iNOS、COX-2和IL-6）的表达水平显著降低。总体而言，我们的结果表明，FAE抑制ROS和紫外线B诱导的NF-κB下游信号通路激活，表明其在药物应用中作为抗自由基损伤的通用佐剂具有相当大的潜力。
