Kalkal Meenu, Chauhan Rubika, Thakur Reva Sharan, Tiwari Mrinalini, Pande Veena, Das Jyoti
Parasite-Host Biology, ICMR-National Institute of Malaria Research, Dwarka, New Delhi 110077, India.
Biotechnology Department, Kumaun University, Nainital 263001, India.
Biology (Basel). 2022 Apr 27;11(5):669. doi: 10.3390/biology11050669.
Various immune cells are known to participate in combating infection. Regulatory B cells represent a subset of B cells that take part in immunomodulation and control inflammation. The immunoregulatory function of regulatory B cells has been shown in various murine models of several disorders. In this study, a comparable IL-10 competent B-10 cell subset (regulatory B cells) was characterized during lethal and non-lethal infection with malaria parasites using the mouse model. We observed that infection of Balb/c mice with I 7XL was lethal, and a rapid increase in dynamics of IL-10 producing B220CD5CD1d regulatory B cells over the course of infection was observed. However, animals infected with a less virulent strain of the parasite I7XNL attained complete resistance. It was observed that there is an increase in the population of regulatory B cells with an increase of parasitemia; however, a sudden drop in the frequency of these cells was observed with parasite clearance. Adoptive transfer of regulatory B cells to naïve mice followed by infection results in slow parasite growth and enhancement of survival in 17XL (lethal) infected animals. Adoptively transferred regulatory B cells also resulted in decreased production of pro-inflammatory cytokine (IFN-γ) and enhanced production of anti-inflammatory cytokine (IL-10). It infers that these regulatory B cells may contribute in immune protection by preventing the inflammation associated with disease and inhibiting the parasite growth.
已知多种免疫细胞参与对抗感染。调节性B细胞是B细胞的一个亚群，参与免疫调节并控制炎症。调节性B细胞的免疫调节功能已在多种疾病的小鼠模型中得到证实。在本研究中，利用小鼠模型，在疟原虫致死性和非致死性感染过程中，对具有类似白细胞介素-10（IL-10）功能的B-10细胞亚群（调节性B细胞）进行了特征分析。我们观察到，用I 7XL感染Balb/c小鼠是致死性的，并且在感染过程中，产生IL-10的B220CD5CD1d调节性B细胞的动态变化迅速增加。然而，感染毒性较小的寄生虫株I7XNL的动物获得了完全抗性。观察到随着寄生虫血症的增加，调节性B细胞的数量增加；然而，随着寄生虫清除，这些细胞的频率突然下降。将调节性B细胞过继转移到未感染的小鼠，随后进行感染，结果导致寄生虫生长缓慢，并提高了17XL（致死性）感染动物的存活率。过继转移的调节性B细胞还导致促炎细胞因子（干扰素-γ）的产生减少，抗炎细胞因子（IL-10）的产生增加。由此推断，这些调节性B细胞可能通过预防与疾病相关的炎症和抑制寄生虫生长来促进免疫保护。
