Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences. 33, Bld. 2 Leninsky Ave., Moscow 119071, Russia.
Biomolecules. 2022 Apr 19;12(5):607. doi: 10.3390/biom12050607.
Chaperones play a vital role in the life of cells by facilitating the correct folding of other proteins and maintaining them in a functional state, being themselves, as a rule, more stable than the rest of cell proteins. Their functional properties naturally tempt investigators to actively adapt them for biotechnology needs. This review will mostly focus on the applications found for the bacterial chaperonin GroE and its counterparts from other organisms, in biotechnology or for research purposes, both in their engineered or intact versions.
伴侣蛋白在细胞的生命中起着至关重要的作用，它们能够促进其他蛋白质的正确折叠，并使它们保持在功能状态，通常情况下，伴侣蛋白比细胞内的其他蛋白质更稳定。它们的功能特性自然吸引了研究人员积极地将它们应用于生物技术需求。本综述主要集中于细菌伴侣蛋白 GroE 及其在生物技术或研究中的应用，包括其工程化或完整版本，以及来自其他生物体的同源物。
