Liu Yuting, Bafaro Elizabeth M, Dempski Robert E
Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Biomolecules. 2022 May 21;12(5):726. doi: 10.3390/biom12050726.
The human (h) transporter hZIP4 is the primary Zn importer in the intestine. hZIP4 is also expressed in a variety of organs such as the pancreas and brain. Dysfunction of hZIP4 can result in the Zn deficiency disease acrodermatitis enteropathica (AE). AE can disrupt digestive and immune system homeostasis. A limited number of hZIP4 expression strategies have hindered increasing knowledge about this essential transmembrane protein. Here, we report the heterologous expression of hZIP4 in . Both a wild-type and a mutant strain, in which the endogenous Zn transporters were deleted, were used to test the expression and localization of an hZIP4-GFP fusion protein. A full-length hZIP4-GFP and a truncated membrane-domain-only (mhZIP4-GFP) protein were observed to be present in the plasma membrane in yeast.
人类（h）转运蛋白hZIP4是肠道中主要的锌导入体。hZIP4也在多种器官中表达，如胰腺和大脑。hZIP4功能失调可导致锌缺乏疾病肠病性肢端皮炎（AE）。AE会破坏消化系统和免疫系统的稳态。hZIP4表达策略的有限性阻碍了对这种重要跨膜蛋白的深入了解。在此，我们报道了hZIP4在……中的异源表达。使用野生型和内源性锌转运体被缺失的突变株来测试hZIP4-GFP融合蛋白的表达和定位。在酵母中观察到全长hZIP4-GFP和仅截短的膜结构域（mhZIP4-GFP）蛋白存在于质膜中。
