Bühler Louisa, Enderle Markus D, Kahn Nicolas, Polke Markus, Schneider Marc A, Heußel Claus Peter, Herth Felix J F, Linzenbold Walter
Erbe Elektromedizin GmbH, 72072 Tübingen, Germany.
Department of Pneumology and Respiratory Care Medicine, Thoraxklinik at Heidelberg University Hospital, 69126 Heidelberg, Germany.
Biomedicines. 2022 May 10;10(5):1100. doi: 10.3390/biomedicines10051100.
(1) Development of radiofrequency ablation (RFA) systems for pulmonary lesions is restricted by availability of human tumor specimens and limited comparability of animal tissue. We aimed to develop a new surrogate tissue overcoming these drawbacks. (2) Reference values for electrical impedance in lung tumor tissue were collected during routine lung tumor RFA (n = 10). Subsequently, a tissue-mimicking surrogate with comparable electrical impedance and facilitating detection of the ablation margins was developed. (3) The mean electrical impedance for all patients was 103.5 ± 14.7 Ω. In the optimized surrogate tissue model consisting of 68% agar solution, 23% egg yolk, 9% thermochromic ink, and variable amounts of sodium chloride, the mean electrical impedance was adjustable from 74.3 ± 0.4 Ω to 183.2 ± 5.6 Ω and was a function (y = 368.4x + 175.2; R2 = 0.96; p < 0.001) of sodium chloride concentration (between 0 and 0.3%). The surrogate tissue achieved sufficient dimensional stability, and sample cuts revealed clear margins of color change for temperatures higher 60 °C. (4) The tissue-mimicking surrogate can be adapted to lung tumor with respect to its electrical properties. As the surrogate tissue allows for simple and cost-effective manufacturing, it is suitable for extensive laboratory testing of RFA systems for pulmonary ablation.
(1) 用于肺部病变的射频消融（RFA）系统的开发受到人类肿瘤标本可用性和动物组织可比性有限的限制。我们旨在开发一种克服这些缺点的新型替代组织。(2) 在常规肺肿瘤RFA期间收集肺肿瘤组织中电阻抗的参考值（n = 10）。随后，开发了一种具有可比电阻抗并有助于检测消融边缘的组织模拟替代物。(3) 所有患者的平均电阻抗为103.5 ± 14.7 Ω。在由68%琼脂溶液、23%蛋黄、9%热致变色墨水和可变数量的氯化钠组成的优化替代组织模型中，平均电阻抗可从74.3 ± 0.4 Ω调节至183.2 ± 5.6 Ω，并且是氯化钠浓度（0至0.3%之间）的函数（y = 368.4x + 175.2；R2 = 0.96；p < 0.001）。替代组织具有足够的尺寸稳定性，样本切片显示温度高于60°C时颜色变化的清晰边缘。(4) 这种组织模拟替代物在电学性质方面可以适应肺肿瘤。由于替代组织允许简单且经济高效的制造，它适用于肺消融RFA系统的广泛实验室测试。
