Li Shan-Shan, Chang Ying, Li Bo, Shao Shu-Li, Zhen-Zhu- Zhang
Qiqihar University, College of Life Sciences and Agriculture and Forestry, Qiqihar, China.
Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, China.
Genet Mol Biol. 2020 May 20;43(2):e20180355. doi: 10.1590/1678-4685-GMB-2018-0355. eCollection 2020.
4-Coumaric acid: coenzyme A ligase (4CL) is a key enzyme in the phenylpropanoid metabolic pathway that regulates the biosynthesis of lignin and flavonoids. Therefore, the study of 4CL is important to explore the accumulation and regulation of metabolites. This study investigated the role that the 4CL2 gene from Dryopteris fragrans (Df4CL2) plays in the metabolite synthesis. Changes in gene expression, enzyme activity, and the content of lignin and flavonoids were measured in different tissues of tobacco as model plant that was successfully transferred with Df4CL2. Tobacco plants with Df4CL2 (transgenic tobacco, TT) were successfully obtained via the Agrobacterium-transformation method. This TT tended to be thicker and had an earlier flowering period than wild type tobacco (WT). The expression levels of Df4CL2 were higher in the stem, leaf, and root in TT compared to WT. In addition, compared to WT, TT had higher 4CL enzyme activity and higher lignin and flavonoids contents. This suggests that Df4CL2 is involved in the synthesis of lignin and flavonoids in D. fragrans. This research provides important evidence toward understanding the phenylpropanoid metabolic pathway in ferns.
4-香豆酸：辅酶A连接酶（4CL）是苯丙烷类代谢途径中的关键酶，该途径调控木质素和类黄酮的生物合成。因此，研究4CL对于探索代谢产物的积累和调控具有重要意义。本研究调查了来自香鳞毛蕨的4CL2基因（Df4CL2）在代谢产物合成中所起的作用。在成功转入Df4CL2的作为模式植物的烟草的不同组织中，测定了基因表达、酶活性以及木质素和类黄酮含量的变化。通过农杆菌转化法成功获得了携带Df4CL2的烟草植株（转基因烟草，TT）。与野生型烟草（WT）相比，这种转基因烟草往往更粗壮，花期更早。与WT相比，TT中Df4CL2在茎、叶和根中的表达水平更高。此外，与WT相比，TT具有更高的4CL酶活性以及更高的木质素和类黄酮含量。这表明Df4CL2参与了香鳞毛蕨中木质素和类黄酮的合成。该研究为理解蕨类植物的苯丙烷类代谢途径提供了重要证据。
