Center for Medical Research, University Hospital, Eberhard Karls University, 72074 72072 Tuebingen, Germany.
Department of Urology, University of Tuebingen Hospital, 72076 Tuebingen, Germany.
Int J Mol Sci. 2022 May 13;23(10):5453. doi: 10.3390/ijms23105453.
Bladder cancer is the most cost-intensive cancer due to high recurrence rates and long follow-up times. Bladder cancer organoids were considered interesting tools for investigating better methods for the detection and treatment of this cancer.
Organoids were generated from urothelial carcinoma tissue samples, then expanded and characterized; the expression of immune modulatory antigens and tumor stem cells markers CD24 and CD44 was explored in early (P ≤ 3) and later (P ≥ 5) passages (P) by immunofluorescence and by quantitative PCR of cDNA. The expression of these factors was investigated in the corresponding cancer tissue samples by immunohistochemistry.
The expression of the PD-L1 was detected on some but not all organoids. CD276 and CD47 were observed on organoids in all passages investigated. Organoids growing beyond passage 8 expressed both CD24 and CD44 at elevated levels in early and late cultures. Organoids proliferating to the eighth passage initially expressed both CD24 and CD44, but lost CD24 expression over time, while CD44 remained. Organoids growing only up to the 6th passage failed to express CD24 but expressed CD44.
The data indicate that the expression of CD24 in urothelial cancer cell organoids may serve as an indicator for the prolonged proliferation potential of the cells.
膀胱癌由于高复发率和长时间的随访，是成本最高的癌症之一。膀胱癌类器官被认为是研究更好的检测和治疗这种癌症方法的有趣工具。
从尿路上皮癌组织样本中生成类器官，然后进行扩增和特征分析；通过免疫荧光和 cDNA 的定量 PCR 探索早期（P ≤ 3）和晚期（P ≥ 5）传代（P）时免疫调节抗原和肿瘤干细胞标志物 CD24 和 CD44 的表达。通过免疫组织化学研究这些因素在相应的癌组织样本中的表达。
在一些但不是所有的类器官上检测到 PD-L1 的表达。在所有研究的传代中，CD276 和 CD47 均在类器官上观察到。超过第 8 代的类器官在早期和晚期培养物中均高水平表达 CD24 和 CD44。增殖至第 8 代的类器官最初表达 CD24 和 CD44，但随着时间的推移，CD24 表达逐渐丢失，而 CD44 保持不变。只能增殖到第 6 代的类器官未能表达 CD24，但表达 CD44。
数据表明，尿路上皮癌细胞类器官中 CD24 的表达可作为细胞长期增殖潜力的指标。
