Department of Urology, Tuebingen University Hospital, 72076 Tübingen, Germany.
Center for Medical Research, University of Tuebingen, 72074 Tübingen, Germany.
Cells. 2023 Aug 31;12(17):2188. doi: 10.3390/cells12172188.
This study investigates the feasibility of establishing urine-derived tumor organoids from bladder cancer (BC) patients as an alternative to tissue-derived organoids. BC is one of the most common cancers worldwide and current diagnostic methods involve invasive procedures. Here, we investigated the potential of using urine samples, which contain exfoliated tumor cells, to generate urine-derived BC organoids (uBCOs). Urine samples from 29 BC patients were collected and cells were isolated and cultured in a three-dimensional matrix. The establishment and primary expansion of uBCOs were successful in 83% of the specimens investigated. The culturing efficiency of uBCOs was comparable to cancer tissue-derived organoids. Immunohistochemistry and immunofluorescence to characterize the uBCOs exhibited similar expressions of BC markers compared to the parental tumor. These findings suggest that urine-derived BC organoids hold promise as a non-invasive tool for studying BC and evaluating therapeutic responses. This approach could potentially minimize the need for invasive procedures and provide a platform for personalized drug screening. Further research in this area may lead to improved diagnostic and treatment strategies for BC patients.
本研究旨在探讨从膀胱癌（BC）患者的尿液中建立肿瘤类器官以替代组织来源的类器官的可行性。BC 是全球最常见的癌症之一，目前的诊断方法涉及有创性操作。在这里，我们研究了利用尿液样本（其中包含脱落的肿瘤细胞）生成尿液源性 BC 类器官（uBCOs）的潜力。收集了 29 名 BC 患者的尿液样本，并在三维基质中分离和培养细胞。在所研究的标本中，83%成功建立并初步扩增了 uBCOs。uBCOs 的培养效率与癌症组织来源的类器官相当。通过免疫组织化学和免疫荧光对 uBCOs 进行特征分析，与亲本肿瘤相比，uBCOs 表现出相似的 BC 标志物表达。这些发现表明，尿液源性 BC 类器官有望成为一种非侵入性工具，用于研究 BC 并评估治疗反应。这种方法可能会最大限度地减少对有创性操作的需求，并为个性化药物筛选提供平台。该领域的进一步研究可能会为 BC 患者带来改进的诊断和治疗策略。
