School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Int J Mol Sci. 2022 May 16;23(10):5551. doi: 10.3390/ijms23105551.
Brassinosteroid-related genes are involved in regulating plant growth and stress responses. However, systematic analysis is limited to Gramineae species, and their roles in plant architecture and salt stress remain unclear. In this study, we identified brassinosteroid-related genes in wheat, barley, maize, and sorghum and investigated their evolutionary relationships, conserved domains, transmembrane topologies, promoter sequences, syntenic relationships, and gene/protein structures. Gene and genome duplications led to considerable differences in gene numbers. Specific domains were revealed in several genes (i.e., , , and ), indicating diverse functions. Protein-protein interactions suggested their synergistic functions. Their expression profiles were investigated in wheat and maize, which indicated involvement in adaptation to stress and regulation of plant architecture. Several candidate genes for plant architecture ( and ) and salinity resistance (, , and ) were identified. This study is the first to comprehensively investigate brassinosteroid-related plant architecture genes in four Gramineae species and should help elucidate the biological roles of brassinosteroid-related genes in crops.
植物激素相关基因参与调控植物生长和应激反应。然而，系统分析仅限于禾本科物种，其在植物结构和盐胁迫方面的作用尚不清楚。在这项研究中，我们鉴定了小麦、大麦、玉米和高粱中的植物激素相关基因，并研究了它们的进化关系、保守结构域、跨膜拓扑结构、启动子序列、基因/蛋白质结构和基因/蛋白质结构的共线性关系。基因和基因组加倍导致基因数量的显著差异。一些基因（如 、 、和 ）中存在特定的结构域，表明其具有多样化的功能。蛋白质-蛋白质相互作用表明它们具有协同作用。我们还研究了小麦和玉米中这些基因的表达模式，结果表明它们参与了对胁迫的适应和对植物结构的调控。鉴定了几个与植物结构（ 、 ）和耐盐性（ 、 、和 ）相关的候选基因。本研究首次全面研究了四个禾本科物种中植物激素相关的植物结构基因，有助于阐明植物激素相关基因在作物中的生物学功能。
