Departamento de Biomedicina Experimental, Facultad de Medicina y Cirugía, Universidad Autónoma "Benito Juárez" de Oaxaca, Oaxaca 68120, Mexico.
Consejo Nacional de Ciencia y Tecnología, Facultad de Medicina y Cirugía de la Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, Mexico.
Int J Mol Sci. 2022 May 18;23(10):5667. doi: 10.3390/ijms23105667.
Platelets play a significant role in hemostasis and perform essential immune functions, evidenced by the extensive repertoire of antimicrobial molecules. Currently, there is no clear description of the presence of azurocidin in human platelets. Azurocidin is a 37 kDa cationic protein abundant in neutrophils, with microbicidal, opsonizing, and vascular permeability-inducing activity. Therefore, this work aimed to characterize the content, secretion, translation, and functions of azurocidin in platelets. Our results show the presence of azurocidin mRNA and protein in α-granules of platelet and megakaryoblasts, and stimulation with thrombin, ADP, and LPS leads to the secretion of free azurocidin as well as within extracellular vesicles. In addition, platelets can translate azurocidin in a basal or thrombin-induced manner. Finally, we found that the addition of low concentrations of azurocidin prevents platelet aggregation and activation. In conclusion, we demonstrate that platelets contain, secrete, and translate azurocidin, and this protein may have important implications for hemostasis.
血小板在止血和发挥重要免疫功能方面发挥着重要作用，这一点可以从其丰富的抗菌分子库中得到证明。目前，关于人类血小板中是否存在天青杀素尚无明确描述。天青杀素是一种 37 kDa 的阳离子蛋白，在中性粒细胞中含量丰富，具有杀菌、调理和血管通透性诱导活性。因此，本研究旨在表征血小板中天青杀素的含量、分泌、翻译和功能。我们的研究结果表明，血小板和巨核细胞前体细胞的α-颗粒中存在天青杀素 mRNA 和蛋白，凝血酶、ADP 和 LPS 的刺激导致游离天青杀素的分泌以及细胞外囊泡中天青杀素的分泌。此外，血小板可以以基础或凝血酶诱导的方式翻译天青杀素。最后，我们发现添加低浓度的天青杀素可以防止血小板聚集和激活。总之，我们证明了血小板含有、分泌和翻译天青杀素，这种蛋白质可能对止血有重要意义。
