Máté Gábor, Balló András, Márk László, Czétány Péter, Szántó Árpád, Török Attila
Pannon Reproduction Institute, 8300 Tapolca, Hungary.
Urology Clinic, University of Pécs Clinical Centre, 7621 Pécs, Hungary.
J Clin Med. 2022 May 21;11(10):2914. doi: 10.3390/jcm11102914.
According to some statistics, absolute asthenozoospermia affects every 1 in 5000 men. Although this incidence rate does not appear to be too high, it is extremely important to address the phenomenon because it can drastically reduce the chances of pregnancy, even with assisted reproduction. The biggest problem with absolute asthenozoospermia is that it is difficult to distinguish between live and dead sperm cells, and fertilization with non-viable spermatozoa may contribute to the failure of an assisted reproduction cycle. Nowadays, DNA fragmentation (DF) is a crucial parameter of semen analysis, and in this paper, we provide evidence of the correlation between DF and vitality. For this purpose, the main semen parameters were investigated by a CASA system (concentration, motility, progressive motility, vitality and DF). In the necrozoospermic group (vitality < 58%), all the measured parameters showed significant differences compared to normal vitality. Concentration (30.1 M mL−1 vs. 13.6 M mL−1), motility (31.9% vs. 18.3%), and progressive motility (24.3% vs. 12.7%) were significantly decreased, while DF was significantly increased (17.4% vs. 23.7%). Based on the connection between vitality decrement and DF increment, DF lowering methods, such as magnetic-activated cell sorting, have been hypothesized as novel methods for the elimination of dead spermatozoa.
根据一些统计数据，每5000名男性中就有1人受绝对弱精子症影响。尽管这一发病率似乎不算太高，但解决这一现象极为重要，因为即使采用辅助生殖技术，它也会大幅降低怀孕几率。绝对弱精子症最大的问题在于难以区分活精子细胞和死精子细胞，而使用无活力的精子进行受精可能会导致辅助生殖周期失败。如今，DNA碎片化（DF）是精液分析的一个关键参数，在本文中，我们提供了DF与活力之间相关性的证据。为此，通过计算机辅助精子分析系统（CASA系统）研究了精液的主要参数（浓度、活力、前向运动能力、活力和DF）。在死精子症组（活力<58%）中，与正常活力相比，所有测量参数均显示出显著差异。浓度（30.1M/mL对13.6M/mL）、活力（31.9%对18.3%）和前向运动能力（24.3%对12.7%）显著降低，而DF显著升高（17.4%对23.7%）。基于活力下降与DF增加之间的联系，诸如磁性激活细胞分选等降低DF的方法已被假定为消除死精子的新方法。
