Artemyeva Marina S, Vasileva Ludmila B, Ma Yi, Kondratov Kirill A, Fedorov Anton V, Kostareva Anna A, Lapshina Sofia E, Anopova Anna D, Likhonosov Nikolai P, Neymark Alexander E, Babenko Alina Yu, Shlyakhto Evgeny V
Federal State Budgetary Institution "V.A. Almazov National Medical Research Centre" of the Ministry of Health of the Russian Federation, 197341 Saint Petersburg, Russia.
Life (Basel). 2022 Apr 25;12(5):633. doi: 10.3390/life12050633.
Bariatric surgery represents a widespread approach to treating morbid obesity. The search for biomarkers to identify patients to whom this type of treatment will be most effective is needed. Our aim was to characterize the relationship of levels of lncRNA H19 in plasma and different adipose tissue depots with patients' response to bariatric surgery. The study includes control subjects, patients with obesity and patients with obesity accompanied by impaired carbohydrate metabolism (ICM). Quantitative analysis of lncRNA H19 levels has been performed using qPCR in plasma and subcutaneous (SAT) and visceral adipose tissue (VAT). Patients with obesity without ICM have higher levels of lncRNA H19 in VAT compared to SAT, and higher levels of lncRNA H19 in SAT compared to SAT of control individuals. One year after the intervention, levels of lncRNA H19 decreased in SAT of patients with obesity without ICM. The preoperative level of lncRNA H19 in VAT demonstrates a positive correlation with excess weight loss and a negative correlation with initial BMI. In conclusion, ICM affects expression of lncRNA H19 in SAT of patients with obesity. The preoperative level of lncRNA H19 in VAT can be used to predict excess weight loss in patients with obesity after bariatric surgery.
减肥手术是治疗病态肥胖的一种广泛采用的方法。需要寻找生物标志物来识别对这种治疗最有效的患者。我们的目的是描述血浆和不同脂肪组织库中lncRNA H19水平与患者对减肥手术反应之间的关系。该研究包括对照受试者、肥胖患者和伴有糖代谢受损（ICM）的肥胖患者。已使用qPCR对血浆、皮下脂肪组织（SAT）和内脏脂肪组织（VAT）中的lncRNA H19水平进行了定量分析。与SAT相比，无ICM的肥胖患者VAT中的lncRNA H19水平更高，与对照个体的SAT相比，肥胖患者SAT中的lncRNA H19水平更高。干预一年后，无ICM的肥胖患者SAT中的lncRNA H19水平下降。VAT中lncRNA H19的术前水平与超重减轻呈正相关，与初始BMI呈负相关。总之，ICM影响肥胖患者SAT中lncRNA H19的表达。VAT中lncRNA H19的术前水平可用于预测减肥手术后肥胖患者的超重减轻情况。
