Parvin Farhana, Vickery Karen, Deva Anand K, Hu Honghua
Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, Australia.
Materials (Basel). 2022 May 19;15(10):3630. doi: 10.3390/ma15103630.
Topical antiseptics are often used to treat chronic wounds with biofilm infections and during salvage of biofilm contaminated implants, but their antibacterial efficacy is frequently only tested against non-aggregated planktonic or free-swimming organisms. This study evaluated the antibacterial and antibiofilm efficacy of four commercial surgical washes Bactisure, TorrenTX, minimally invasive lavage (MIS), and Betadine against six bacterial species: , , , , and which are commonly isolated from surgical site infections and chronic wound infections using different in vitro models. We determined minimum planktonic inhibitory and eradication concentration and minimum 1-day-old biofilm inhibition and eradication concentration of antiseptics in 96-well plates format with 24 h contact time. We also tested the efficacy of antiseptics at in-use concentration and contact time in the presence of biological soil against 3-day-old biofilm grown on coupons with shear in a bioreactor, such that the results are more applicable to the clinical biofilm situations. In the 96-well plate model, the minimum concentration required to inhibit or kill planktonic and biofilm bacteria was lower for Bactisure and TorrenTX than for MIS and Betadine. However, Betadine and Bactisure showed better antibiofilm efficacy than TorrenTX and MIS in the 3-day-old biofilm bioreactor model at in-use concentration. The minimal concentration of surgical washes required to inhibit or kill planktonic bacterial cells and biofilms varies, suggesting the need for the development and use of biofilm-based assays to assess antimicrobial therapies, such as topical antiseptics and their effective concentrations. The antibiofilm efficacy of surgical washes against different bacterial species also varies, highlighting the importance of testing against various bacterial species to achieve a thorough understanding of their efficacy.
局部抗菌剂常用于治疗伴有生物膜感染的慢性伤口以及在挽救被生物膜污染的植入物时使用，但它们的抗菌效果通常仅针对非聚集的浮游或自由游动生物进行测试。本研究使用不同的体外模型，评估了四种市售手术冲洗液Bactisure、TorrenTX、微创灌洗（MIS）和碘伏对六种细菌的抗菌和抗生物膜效果，这六种细菌分别为金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌和鲍曼不动杆菌，它们通常从手术部位感染和慢性伤口感染中分离出来。我们在96孔板中以24小时接触时间测定了抗菌剂的最低浮游抑制和根除浓度以及最低1日龄生物膜抑制和根除浓度。我们还测试了在使用浓度和接触时间下，抗菌剂在存在生物污垢的情况下对在生物反应器中带有剪切力的试片上生长的3日龄生物膜的效果，以使结果更适用于临床生物膜情况。在96孔板模型中，Bactisure和TorrenTX抑制或杀死浮游和生物膜细菌所需的最低浓度低于MIS和碘伏。然而，在使用浓度下，在3日龄生物膜生物反应器模型中，碘伏和Bactisure显示出比TorrenTX和MIS更好的抗生物膜效果。抑制或杀死浮游细菌细胞和生物膜所需的手术冲洗液的最低浓度各不相同，这表明需要开发和使用基于生物膜的检测方法来评估抗菌疗法，如局部抗菌剂及其有效浓度。手术冲洗液对不同细菌种类的抗生物膜效果也各不相同，这突出了针对各种细菌种类进行测试以全面了解其效果的重要性。
