Kim Ye Jin, Kang Dasol, Yang Hye Rim, Park Byong Seo, Tu Thai Hien, Jeong Bora, Lee Byung Ju, Kim Jae Kwang, Kim Jae Geun
Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Korea.
Department of Biological Science, University of Ulsan, Ulsan 44610, Korea.
Metabolites. 2022 Apr 29;12(5):407. doi: 10.3390/metabo12050407.
Nutrient availability and utilization in hypothalamic cells are directly associated with the regulation of whole-body energy homeostasis. Thus, establishing metabolic profiling in the hypothalamus in response to metabolic shift is valuable to better understand the underlying mechanism of appetite regulation. In the present study, we evaluate the alteration of lipophilic and hydrophilic metabolites in both the hypothalamus and serum of fasted mice. Fasted mice displayed an elevated ketone body and decreased lactate levels in the hypothalamus. In support of the metabolite data, we further confirmed that short-term food deprivation resulted in the altered expression of genes involved in cellular metabolic processes, including the shuttling of fuel sources and the production of monocarboxylates in hypothalamic astrocytes. Overall, the current study provides useful information to close the gap in our understanding of the molecular and cellular mechanisms underlying hypothalamic control of whole-body energy metabolism.
下丘脑细胞中的营养物质可用性和利用与全身能量稳态的调节直接相关。因此，建立下丘脑对代谢变化的代谢谱对于更好地理解食欲调节的潜在机制具有重要价值。在本研究中，我们评估了禁食小鼠下丘脑和血清中亲脂性和亲水性代谢物的变化。禁食小鼠下丘脑的酮体水平升高，乳酸水平降低。为支持代谢物数据，我们进一步证实短期食物剥夺导致下丘脑星形胶质细胞中参与细胞代谢过程的基因表达改变，包括燃料来源的穿梭和单羧酸的产生。总体而言，本研究提供了有用信息，以弥补我们在理解下丘脑对全身能量代谢控制的分子和细胞机制方面的差距。
