Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 406-772, Korea.
Department of Nano-Bioengineering, Incheon National University, Incheon 406-772, Korea.
Molecules. 2020 Jul 11;25(14):3170. doi: 10.3390/molecules25143170.
Degenerative diseases, which can develop during aging, are underlined by inflammatory processes. Hypothalamic inflammation triggered by elevation in circulating fatty acid levels is directly coupled to metabolic disorders. The present study aimed to investigate and characterize the hypothalamic inflammation and composition of fatty acids in the hypothalami of aged mice. We verified that inflammation and microglial activation occur in the hypothalami of aged mice by performing quantitative real-time PCR and using immunohistochemistry methods. In addition, we observed increased levels of various saturated fatty acids in the hypothalami of aged mice, whereas no major changes in the levels of circulating fatty acids were detected using gas chromatography with a flame ionization detector. Collectively, our current findings suggest that increases in saturated fatty acid levels are coupled to hypothalamic inflammation and thereby cause perturbations in energy metabolism during the aging process.
退行性疾病可在衰老过程中发生，其特征是炎症过程。循环脂肪酸水平升高引发的下丘脑炎症与代谢紊乱直接相关。本研究旨在研究和描述老年小鼠下丘脑的炎症和脂肪酸组成。我们通过实时定量 PCR 和免疫组织化学方法证实了老年小鼠的下丘脑存在炎症和小胶质细胞激活。此外，我们观察到老年小鼠的下丘脑内各种饱和脂肪酸水平升高，而使用火焰离子化检测器的气相色谱法未检测到循环脂肪酸水平的主要变化。总的来说，我们目前的研究结果表明，饱和脂肪酸水平的增加与下丘脑炎症有关，从而导致衰老过程中能量代谢的紊乱。
