Gu Lihao, Maeda Kazuhisa
Bionics Program, Tokyo University of Technology Graduate School, 1404-1 Katakura-machi, Hachioji City 192-0982, Tokyo, Japan.
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura-machi, Hachioji City 192-0982, Tokyo, Japan.
Metabolites. 2022 May 3;12(5):412. doi: 10.3390/metabo12050412.
We reported that raspberry ketone (RK) is produced from rhododendrol (RD) in excised mouse skin. We confirmed that RK is also produced from RD in human skin homogenates. We also observed more conversion of RD to RK when the oxidized form of nicotinamide adenine dinucleotide (NAD), a coenzyme of alcohol dehydrogenase (ADH), was added to human skin homogenates. Chiral column analysis of the consumption of RD enantiomers in human skin homogenates also showed that more of the R enantiomers of RD remained than the S enantiomers of RD. This suggests that the S-enantiomer of RD is more easily oxidized in human skin. We confirmed that RD is partially metabolized to RK in human skin, thus suggesting that ADH in the skin may be the main cause of the appearance of this oxidation product.
我们报道了在切除的小鼠皮肤中，杜鹃醇（RD）可生成树莓酮（RK）。我们证实，在人皮肤匀浆中，RD也可生成RK。当将酒精脱氢酶（ADH）的辅酶烟酰胺腺嘌呤二核苷酸（NAD）的氧化形式添加到人皮肤匀浆中时，我们还观察到RD向RK的转化率更高。对人皮肤匀浆中RD对映体消耗情况的手性柱分析也表明，剩余的RD的R对映体比S对映体更多。这表明RD的S对映体在人皮肤中更容易被氧化。我们证实，RD在人皮肤中会部分代谢为RK，因此表明皮肤中的ADH可能是这种氧化产物出现的主要原因。
