转录组图谱揭示了与内洛尔牛肌肉中必需脂肪酸含量相关的遗传和代谢机制。

Transcriptome Profile Reveals Genetic and Metabolic Mechanisms Related to Essential Fatty Acid Content of Intramuscular in Nellore Cattle.

作者信息

Schettini Gustavo Pimenta, Peripolli Elisa, Alexandre Pâmela Almeida, Dos Santos Wellington Bizarria, Pereira Angélica Simone Cravo, de Albuquerque Lúcia Galvão, Baldi Fernando, Curi Rogério Abdallah

机构信息

School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal 14884-900, SP, Brazil.

School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga 13635-900, SP, Brazil.

出版信息

Metabolites. 2022 May 23;12(5):471. doi: 10.3390/metabo12050471.

DOI:10.3390/metabo12050471

PMID:35629975

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9144777/

Abstract

Beef is a source of essential fatty acids (EFA), linoleic (LA) and alpha-linolenic (ALA) acids, which protect against inflammatory and cardiovascular diseases in humans. However, the intramuscular EFA profile in cattle is a complex and polygenic trait. Thus, this study aimed to identify potential regulatory genes of the essential fatty acid profile in of Nellore cattle finished in feedlot. Forty-four young bulls clustered in four groups of fifteen animals with extreme values for each FA were evaluated through differentially expressed genes (DEG) analysis and two co-expression methodologies (WGCNA and PCIT). We highlight the , , , and genes and the TF , indicated in both FA. Moreover, we associate the , , , , and genes with LA, and the and genes with ALA. Furthermore, the functional enrichment analysis points out several terms related to FA metabolism. These findings contribute to our understanding of the genetic mechanisms underlying the beef EFA profile in Nellore cattle finished in feedlot.

摘要

牛肉是必需脂肪酸（EFA）、亚油酸（LA）和α-亚麻酸（ALA）的来源，这些脂肪酸可预防人类的炎症性疾病和心血管疾病。然而，牛的肌肉内必需脂肪酸谱是一个复杂的多基因性状。因此，本研究旨在确定在饲养场育肥的内洛尔牛中必需脂肪酸谱的潜在调控基因。通过差异表达基因（DEG）分析和两种共表达方法（WGCNA和PCIT）对44头年轻公牛进行了评估，这些公牛分为四组，每组15头，每组的每种脂肪酸含量都有极端值。我们重点关注了两种脂肪酸中都显示出的、、、和基因以及转录因子。此外，我们将、、、、和基因与亚油酸相关联，将和基因与α-亚麻酸相关联。此外，功能富集分析指出了几个与脂肪酸代谢相关的术语。这些发现有助于我们理解在饲养场育肥的内洛尔牛中牛肉必需脂肪酸谱的遗传机制。