Department of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
Drug Deliv Transl Res. 2021 Jun;11(3):1133-1143. doi: 10.1007/s13346-020-00836-y.
The platform of the combination chemo-photodynamic therapy has received widespread attention for enhancing anticancer efficacy and inhibiting tumor growth, which supports thermosensitive and controlled drug release. Here, an injectable thermoreversible hydrogel (BPNSs/DTX-M-hydrogel) co-encapsulating black phosphorus nanosheets (BPNSs) and docetaxel (DTX) micelles was prepared to increase drug accumulation in tumor tissue and improve anticancer efficacy. BPNSs were prepared by liquid exfoliation method with a simple and rapid preparation, and DTX micelles were prepared by the thin film dispersion method. Hydrogel was prepared with F127 as hydrogel matrix for intratumoral injection. BPNSs, DTX micelles, and BPNSs/DTX-M-hydrogel were characterized by particle size, morphology, stability and degradation, phase transition feature, and photodynamic performance. And the in vivo anticancer efficacy was evaluated in 4T1 tumor-bearing Balb/c mice. The results showed that the particle size of DTX micelles and BPNSs were about 16 and 180 nm, respectively. The hydrogel with the transformation temperature at near body exhibited great photodynamic efficacy and good biodegradability. Moreover, BPNSs/DTX-M-hydrogel with the combination of chemotherapy and photodynamic therapy exhibited unique anticancer efficacy with low toxicity. In conclusion, the combination platform of chemo-photodynamic therapy based on BPNSs could be a prospective strategy in antitumor research. Graphical abstract.
联合化疗-光动力疗法平台因其能增强抗癌疗效和抑制肿瘤生长而受到广泛关注，该平台支持热敏和控制药物释放。在此，我们制备了一种可注射的温敏型可逆水凝胶（BPNSs/DTX-M-水凝胶），其共包封了黑磷纳米片（BPNSs）和多西紫杉醇（DTX）胶束，以增加药物在肿瘤组织中的积累并提高抗癌疗效。BPNSs 通过液相剥离法制备，具有简单快速的制备方法，DTX 胶束通过薄膜分散法制备。水凝胶以 F127 为水凝胶基质，用于瘤内注射。通过粒径、形态、稳定性和降解、相转变特性和光动力性能对 BPNSs、DTX 胶束和 BPNSs/DTX-M-水凝胶进行了表征。并在 4T1 荷瘤 Balb/c 小鼠中评估了体内抗癌疗效。结果表明，DTX 胶束和 BPNSs 的粒径分别约为 16nm 和 180nm。具有近体温相变温度的水凝胶表现出优异的光动力疗效和良好的生物降解性。此外，联合化疗和光动力治疗的 BPNSs/DTX-M-水凝胶具有独特的抗癌疗效和低毒性。总之，基于 BPNSs 的化疗-光动力联合治疗平台可能是抗肿瘤研究的一种有前景的策略。
