基于黑磷纳米片的无标记适体传感器用于检测氟虫腈。

Label-Free Aptasensor for Detection of Fipronil Based on Black Phosphorus Nanosheets.

机构信息

Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.

College of Packing and Materials Engineering, Hunan University of Technology, Zhuzhou 412007, China.

出版信息

Biosensors (Basel). 2022 Sep 20;12(10):775. doi: 10.3390/bios12100775.

A label-free fipronil aptasensor was built based on Polylysine-black phosphorus nanosheets composition (PLL-BPNSs) and Au nanoparticles (AuNPs). A PLL-BP modified glassy carbon electrode (GCE) was fabricated by combining BP NSs and PLL, which included a considerable quantity of -NH. Au nanoparticles (AuNPs) were placed onto the GCE, and PLL-BPNSs bonded to Au NPs firmly by assembling. The thiolated primers were then added and fixed using an S-Au bond, and competitive binding of the fipronil aptamer was utilized for fipronil quantitative assessment. The sensor's performance was evaluated using differential pulse voltammetry (DPV) method. The linear equation is ΔI (μA) = 13.04 logC + 22.35, while linear correlation coefficient R is 0.998, and detection limit is 74 pg/mL (0.17 nM) when the concentration of fipronil is 0.1 ng/mL-10 μg/mL. This aptasensor can apply to quantitative detection of fipronil.

建立了一种基于聚赖氨酸-黑磷纳米片复合物（PLL-BPNSs）和金纳米粒子（AuNPs）的无标记氟虫腈适体传感器。通过将 BP NSs 和 PLL 结合，制备了一种 PLL-BP 修饰的玻碳电极（GCE），其中包含大量的-NH。Au 纳米粒子（AuNPs）被放置在 GCE 上，并且 PLL-BPNSs 通过组装牢固地结合到 Au NPs 上。然后添加巯基化引物并利用 S-Au 键固定，利用氟虫腈适体的竞争结合对氟虫腈进行定量评估。使用差分脉冲伏安法（DPV）方法评估传感器的性能。线性方程为ΔI（μA）= 13.04 logC + 22.35，当氟虫腈的浓度为 0.1 ng/mL-10 μg/mL 时，线性相关系数 R 为 0.998，检测限为 74 pg/mL（0.17 nM）。该适体传感器可用于氟虫腈的定量检测。