Pope Emily, Haltli Bradley, Kerr Russell G, Ahmadi Ali
Department of Biomedical Science, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Nautilus Biosciences Croda, Charlottetown, PE C1A 4P3, Canada.
Microorganisms. 2022 May 10;10(5):996. doi: 10.3390/microorganisms10050996.
To enhance the discovery of novel natural products, various innovations have been developed to aid in the cultivation of previously unculturable microbial species. One approach involving the microencapsulation of bacteria has been gaining popularity as a new cultivation technique, with promising applications. Previous studies demonstrated the success of bacterial encapsulation; however, they highlighted that a key limitation of encapsulating bacteria within agarose is the high temperature required for encapsulation. Encapsulation of bacteria within agarose typically requires a temperature high enough to maintain the flow of agarose through microfluidic devices without premature gelation. Given the sensitivity of many bacterial taxa to temperature, the effect of various agarose-based encapsulating matrices on marine bacterial viability was assessed to further develop this approach to bacterial culture. It was determined that lowering the temperature of encapsulation via the use of low-gelling-temperature agarose, as well as the addition of nutrients to the matrix, significantly improved the viability of representative marine sediment bacteria in terms of abundance and metabolic activity. Based on these findings, the use of low-gelling-temperature agarose with supplemental nutrients is recommended for the encapsulation of marine bacteria obtained from temperate habitats.
为了加强新型天然产物的发现，人们开发了各种创新方法来辅助培养以前无法培养的微生物物种。一种涉及细菌微囊化的方法作为一种新的培养技术越来越受欢迎，具有广阔的应用前景。先前的研究证明了细菌微囊化的成功；然而，这些研究强调，将细菌包裹在琼脂糖内的一个关键限制是包裹所需的高温。在琼脂糖内包裹细菌通常需要足够高的温度，以维持琼脂糖通过微流控装置的流动而不会过早凝胶化。鉴于许多细菌类群对温度敏感，因此评估了各种琼脂糖基包裹基质对海洋细菌活力的影响，以进一步开发这种细菌培养方法。结果表明，通过使用低凝胶温度琼脂糖降低包裹温度，以及在基质中添加营养物质，在丰度和代谢活性方面显著提高了代表性海洋沉积物细菌的活力。基于这些发现，建议使用含有补充营养物质的低凝胶温度琼脂糖来包裹从温带栖息地获得的海洋细菌。
