Department of Experimental and Clinical Pharmacology, Medical University, ul. Jaczewskiego 8b, 20-090 Lublin, Poland.
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka st. 12, 20-950 Lublin, Poland.
Nutrients. 2022 May 14;14(10):2062. doi: 10.3390/nu14102062.
Alpha-ketoglutarate (AKG) is one of the key metabolites that play a crucial role in cellular energy metabolism. Bariatric surgery is a life-saving procedure, but it carries many gastrointestinal side effects. The present study investigated the beneficial effects of dietary AKG on the structure, integrity, and absorption surface of the small intestine after bariatric surgery. Male 7-week-old Sprague Dowley rats underwent gastric bypass surgery, after which they received AKG, 0.2 g/kg body weight/day, administered in drinking water for 6 weeks. Changes in small intestinal morphology, including histomorphometric parameters of enteric plexuses, immunolocalization of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal mucosa, and selected hormones, were evaluated. Proliferation, mucosal and submucosal thickness, number of intestinal villi and Paneth cells, and depth of crypts were increased; however, crypt activity, the absorption surface, the expression of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal epithelium were decreased after gastric bypass surgery. Alpha-ketoglutarate supplementation partially improved intestinal structural parameters and epithelial integrity in rats undergoing this surgical procedure. Dietary AKG can abolish adverse functional changes in the intestinal mucosa, enteric nervous system, hormonal response, and maintenance of the intestinal barrier that occurred after gastric bypass surgery.
α-酮戊二酸（AKG）是一种关键代谢物，在细胞能量代谢中起着至关重要的作用。减重手术是一种救生手术，但它有许多胃肠道副作用。本研究探讨了饮食 AKG 对减重手术后小肠结构、完整性和吸收表面的有益影响。雄性 7 周龄 Sprague Dawley 大鼠接受胃旁路手术后，每天给予 0.2 g/kg 体重的 AKG，通过饮用水给予 6 周。评估了小肠形态的变化，包括肠丛的组织形态学参数、Claudin 3、MarvelD3、occludin 和 zonula ocludens 1 在肠黏膜中的免疫定位，以及选定的激素。胃旁路手术后，增殖、黏膜和黏膜下层厚度、肠绒毛和潘氏细胞数量以及隐窝深度增加；然而，隐窝活性、吸收表面、肠上皮细胞中 Claudin 3、MarvelD3、occludin 和 zonula ocludens 1 的表达减少。AKG 补充部分改善了接受这种手术的大鼠的肠道结构参数和上皮完整性。饮食 AKG 可以消除胃旁路手术后发生的肠道黏膜、肠神经系统、激素反应和肠道屏障维持的不良功能变化。
