Natsheh Hiba, Touitou Elka
The Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.
Pharmaceutics. 2022 Apr 22;14(5):918. doi: 10.3390/pharmaceutics14050918.
Pomegranate seed oil (PSO) is currently administrated orally as a food supplement for improving memory. However, the efficiency of the oral dosage forms for such purposes is low, mainly due to the blood brain barrier impeding a good delivery to brain. In this work, we designed and characterized a PSO phospholipid oily gel for nasal administration. We tested the performance of the new PSO delivery system in animal models for impaired memory and locomotor activity. The experimental results indicated a statistically significant improvement (p < 0.05) of more than 1.5 fold in the behavior of animals treated nasally, in comparison to those treated with orally administrated oil. Furthermore, in multiphoton microscopy and near infrared imaging studies, the nasal administration of fluorescent probes, fluorescein isothiocyanate (FITC), and indocyanine green (ICG) incorporated in the PSO system showed enhanced delivery to the brain. Results of the histopathologic examination of the nasal cavity and mucosa, as carried out by a pathologist, indicated the safety of the PSO phospholipid oily gel. In conclusion, the results of this work encourage further investigation of the phospholipid oily gel composition as a new way of PSO administration.
石榴籽油（PSO）目前作为一种改善记忆力的食品补充剂口服给药。然而，此类口服剂型在这方面的效率较低，主要是因为血脑屏障阻碍了其向大脑的良好递送。在这项工作中，我们设计并表征了一种用于鼻腔给药的PSO磷脂油凝胶。我们在记忆受损和运动活动的动物模型中测试了这种新型PSO递送系统的性能。实验结果表明，与口服油剂治疗的动物相比，鼻腔给药治疗的动物行为有统计学显著改善（p < 0.05），改善幅度超过1.5倍。此外，在多光子显微镜和近红外成像研究中，将荧光探针异硫氰酸荧光素（FITC）和吲哚菁绿（ICG）掺入PSO系统进行鼻腔给药显示向大脑的递送增强。病理学家对鼻腔和黏膜进行的组织病理学检查结果表明PSO磷脂油凝胶是安全的。总之，这项工作的结果鼓励进一步研究磷脂油凝胶组合物作为PSO给药的新途径。
