Rajasekharan Satish Kumar, Shemesh Moshe
Department of Food Science, Institute of Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Institute, Rishon LeZion 7528809, Israel.
Pharmaceutics. 2022 Apr 29;14(5):962. doi: 10.3390/pharmaceutics14050962.
Secreted molecules from probiotic have often been considered potential pharmaceuticals to fight infections caused by bacterial or yeast pathogens. In the present study, we investigated the antagonistic potential of secreted probiotic filtrates (hereafter, postbiotics) derived from cells against pathogenic microorganisms, such as , , and . We found that the postbiotics mitigate the biofilms of the tested pathogens with no notable effect on their planktonic growth. In addition, the postbiotics suppressed some virulence traits, for instance, the dendrite swarming motility of and yeast-to-hyphal switch in . Further assays with an active constituent produced by the cells-2-undecanone revealed two significant findings: (i) 2-undecanone inhibits biofilms and hyphae and in a model, and (ii) it interacts specifically with Gln 58 amino acid residue of hyphal wall protein-1 (Hwp-1) in molecular docking analysis. The results suggest the targeted mode of antagonistic action of 2-undecanone against biofilm. In total, the findings of the study depict an appealing strategy to use postbiotics, including specific ketone molecules, produced by for developing novel antibiofilm and anti-hyphal pharmaceuticals.
益生菌分泌的分子常被视为对抗细菌或酵母病原体引起感染的潜在药物。在本研究中，我们调查了源自[具体菌株]细胞的益生菌分泌滤液（以下简称后生元）对[具体病原体1]、[具体病原体2]和[具体病原体3]等致病微生物的拮抗潜力。我们发现后生元可减轻受试病原体的生物膜，对其浮游生长无显著影响。此外，后生元抑制了一些毒力特性，例如[具体病原体1]的树突状群体运动性和[具体病原体2]的酵母-菌丝转变。对[具体菌株]细胞产生的活性成分2-十一烷酮的进一步分析揭示了两个重要发现：（i）2-十一烷酮在[具体模型]中抑制[具体病原体1]的生物膜和菌丝，（ii）在分子对接分析中，它与菌丝壁蛋白-1（Hwp-1）的Gln 58氨基酸残基特异性相互作用。结果表明2-十一烷酮对[具体病原体1]生物膜的靶向拮抗作用模式。总体而言，该研究结果描述了一种有吸引力的策略，即利用[具体菌株]产生的后生元，包括特定的酮分子，来开发新型抗生物膜和抗菌丝药物。
